Location of the Cell Adhesion Molecule "Coxsackievirus and Adenovirus Receptor" in the Adult Mouse Brain
Location of the Cell Adhesion Molecule "Coxsackievirus and Adenovirus Receptor" in the Adult Mouse Brain
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DOI：
10.3389/fnana.2020.00028
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发表时间：
2020-06-04
影响因子：
2.9
通讯作者：
Dopeso-Reyes, Iria G.
中科院分区：
文献类型：
--
作者：
Wehbi, Amani;Kremer, Eric J.;Dopeso-Reyes, Iria G.
柯萨奇病毒和腺病毒受体（CAR）是一种单次跨膜细胞粘附分子（CAM）。 CAR 在许多哺乳动物组织中表达，包括大脑、心脏、肺和睾丸。在上皮细胞中，CAR 功能是众多 CAM 的典型典型作用。然而，人们对 CAR 在大脑中的多种作用知之甚少。为了更好地了解 CAR 在成人大脑中的生理作用，描述其位置是推进我们对其功能了解的首要步骤。此外，CAR 负责犬腺病毒 2 型 (CAV-2) 载体的附着、内化和逆行运输，该载体在神经元回路图谱和基因转移以治疗和模拟神经退行性疾病方面找到了利基。在这项研究中，我们使用免疫组织化学和免疫荧光来记录 CAR 在健康年轻成年小鼠大脑中的整体位置。在全球范围内，我们发现 CAR 由脑实质中正在成熟的神经元表达，并位于胞体和突起上。虽然 CAR 偶尔与神经胶质原纤维酸性蛋白共定位，但这种重叠仅限于与成人神经发生相关的区域。
The coxsackievirus and adenovirus receptor (CAR) is a single-pass transmembrane cell adhesion molecule (CAM). CAR is expressed in numerous mammalian tissues including the brain, heart, lung, and testes. In epithelial cells, CAR functions are typical of the quintessential roles of numerous CAMs. However, in the brain the multiple roles of CAR are poorly understood. To better understand the physiological role of CAR in the adult brain, characterizing its location is a primordial step to advance our knowledge of its functions. In addition, CAR is responsible for the attachment, internalization, and retrograde transport of canine adenovirus type 2 (CAV-2) vectors, which have found a niche in the mapping of neuronal circuits and gene transfer to treat and model neurodegenerative diseases. In this study, we used immunohistochemistry and immunofluorescence to document the global location of CAR in the healthy, young adult mouse brain. Globally, we found that CAR is expressed by maturing and mature neurons in the brain parenchyma and located on the soma and on projections. While CAR occasionally colocalizes with glial fibrillary acidic protein, this overlap was restricted to areas that are associated with adult neurogenesis.