The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I.
The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I.
复制标题
志贺氏菌腹泻的发病机制。
DOI：
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发表时间：
1972
影响因子：
15.9
通讯作者：
J. Mciver
中科院分区：
文献类型：
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作者：
G. Keusch;G. Grady;L. Mata;J. Mciver
1969年8月，从危地马拉一名痢疾患者身上新分离出的志贺氏痢疾杆菌1型菌株被发现在肉汤培养液中制造了一种肠毒素。通过在分级聚合膜和Sephadex凝胶过滤上的超滤对肠毒素进行部分纯化（Pharmacia Fine Chemicals, Inc., Piscataway, n.j.）表明其分子量约为55,000-60,000。部分纯化的毒素对热不稳定，对酶敏感，受碱性pH激活，在结扎的家兔回肠段产生液体；然而，它未能引起皮肤血管通透性的增加。当同样重量制备的霍乱弧菌和痢疾杆菌肠毒素在家兔回肠中的活性比较时，随着钾、氯化物和蛋白质浓度的增加，后者引起的体积反应明显较小。先前描述的神经毒性（小鼠致死）因子也存在，并与肠毒素一起从Sephadex G-150中洗脱。如果这些生物活性被证明是由单一分子物种所具有的，建议将其重新命名为志贺氏菌肠毒素，以识别生理上更相关的生物作用。
A strain of Shigella dysenteriae 1, freshly isolated from a patient with dysentery in Guatemala in August 1969, was found to elaborate an enterotoxin into the liquid of broth cultures. Partial purification of the enterotoxin by ultrafiltration on graded polymeric membranes and Sephadex gel filtration (Pharmacia Fine Chemicals, Inc., Piscataway, N. J.) suggested an approximate molecular weight of 55,000-60,000. The partially purified toxin was heat-labile, pronase sensitive, and activated by alkaline pH, and it elicited fluid production in ligated rabbit ileal segments; it failed, however, to cause increased vascular permeability in skin. When the activities of equal weights of identically prepared Vibrio cholerae and S. dysenteriae enterotoxins were compared in the rabbit ileum the latter caused a significantly smaller volume response with increased concentrations of potassium, chloride, and protein. The previously described neurotoxic (mouse lethal) factor was also present and eluted from Sephadex G-150 with the enterotoxin. If these biological activities prove to be possessed by a single molecular species, it is suggested that it be renamed Shigella enterotoxin in recognition of the physiologically more relevant biological action.