Formation of dAMP-glycerol and dAMP-Tris Derivatives by Thermococcus kodakaraensis DNA Primase
Formation of dAMP-glycerol and dAMP-Tris Derivatives by Thermococcus kodakaraensis DNA Primase
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DOI：
10.1074/jbc.m111.338160
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发表时间：
2012-05-11
影响因子：
4.8
通讯作者：
Hurwitz, Jerard
中科院分区：
文献类型：
--
作者：
Galal, Wiebke Chemnitz;Pan, Miao;Hurwitz, Jerard
在dATP、甘油和Tris缓冲液存在下，从Thermococcus kodakaraensis分离的DNA引发酶催化dAMP和两种产物的形成，其被鉴定为dAMP-甘油和dAMP-Tris。这些产物是由T. kodakaraensis p41催化亚基单独和T.在不存在DNA模板的情况下，使用p41-p46复合物来表达。它们不是用含有无催化活性的p41亚基的制剂形成的。类似的甘油和Tris衍生物以及dNMP也与dGTP、dCTP或dTTP形成。本文还讨论了这些产物的形成机理及其在T。Kodakaraensis引发酶。
In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis catalyzed the formation of dAMP and two products that were identified as dAMP-glycerol and dAMP-Tris. These products were formed by the T. kodakaraensis p41 catalytic subunit alone and the T. kodakaraensis p41-p46 complex in the absence of a DNA template. They were not formed with preparations containing the catalytically inactive p41 subunit. Similar glycerol and Tris derivatives as well as dNMPs were also formed with dGTP, dCTP, or dTTP. The mechanism contributing to the formation of these products and its implications in the initiation reaction catalyzed by the T. kodakaraensis primase are discussed.