NACL REGULATION OF PLASMA-MEMBRANE H+-ATPASE GENE-EXPRESSION IN A GLYCOPHYTE AND A HALOPHYTE
NACL REGULATION OF PLASMA-MEMBRANE H+-ATPASE GENE-EXPRESSION IN A GLYCOPHYTE AND A HALOPHYTE
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DOI：
10.1104/pp.103.3.713
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发表时间：
1993-11-01
期刊：
影响因子：
7.4
通讯作者：
HASEGAWA, PM
中科院分区：
文献类型：
--
作者：
NIU, XM;NARASIMHAN, ML;HASEGAWA, PM
NaCl对糖基植物烟草质膜H ~+-ATPase基因表达的调控var威斯康星州38）和盐生植物滨藜Atriplex nummularia L.通过使用编码各植物ATP酶的同源cDNA探针比较器官特异性mRNA丰度来评估。NaCl诱导的mRNA积累在完全展开的叶和根，但不是在扩展的叶或茎。盐生植物根系积累质膜H ~+-ATPase mRNA的NaCl响应性明显大于糖生植物。盐诱导的A.原位杂交结果显示，洋地黄根主要位于伸长区，但mRNA水平在分化区也有所增加。增加了A中的信息积累。在NaCl（400 mM）处理后8 h内可检测到nummularia根的最大浓度，其最大浓度是未处理对照植物根的几倍。NaCl诱导的质膜H+-ATP酶基因的表达在扩大的叶和根推测表明，这些器官需要增加H+-电化学电位梯度的植物离子稳态盐适应的维护。盐生植物在NaCl胁迫下诱导质膜H+-ATPase基因表达的能力增强可能是其耐盐性的决定因素。
NaCl regulation of plasma membrane H+-ATPase gene expression in the glycophyte tobacco (Nicotiana tabacum L. var Wisconsin 38) and the halophyte Atriplex nummularia L. was evaluated by comparison of organ-specific mRNA abundance using homologous cDNA probes encoding the ATPases of the respective plants. Accumulation of mRNA was induced by NaCl in fully expanded leaves and in roots but not in expanding leaves or stems. The NaCl responsiveness of the halophyte to accumulate plasma membrane H+-ATPase mRNA in roots was substantially greater than that of the glycophyte. Salt-induced transcript accumulation in A. nummularia roots was localized by in situ hybridization predominantly to the elongation zone, but mRNA levels also increased in the zone of differentiation. Increased message accumulation in A. nummularia roots could be detected within 8 h after NaCl (400 mM) treatment, and maximal levels were severalfold greater than in roots of untreated control plants. NaCl-induced plasma membrane H+-ATPase gene expression in expanded leaves and roots presumably indicates that these organs require increased H+-electrochemical potential gradients for the maintenance of plant ion homeostasis for salt adaptation. The greater capacity of the halophyte to induce plasma membrane H+-ATPase gene expression in response to NaCl may be a salt-tolerance determinant.