Tear and serum MMP-9 and serum TIMPs levels in the severe sulfur mustard eye injured exposed patients
Tear and serum MMP-9 and serum TIMPs levels in the severe sulfur mustard eye injured exposed patients
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DOI：
10.1016/j.intimp.2019.105812
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发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Ghazanfari, Tooba
中科院分区：
文献类型：
--
作者：
Ghasemi, Hassan;Yaraee, Roya;Ghazanfari, Tooba
简介：硫芥（SM）中毒会引起人体局部和全身的变化。在这项研究中，泪液和血清基质金属蛋白酶（MMP）-9和血清金属蛋白酶组织抑制剂（TIMPs）的关系进行了评估严重眼外伤SM暴露countries.Methods：一组128例严重眼外伤患者分为三个亚组（轻度19例，中度31例，重度78例）与31名健康对照组进行了比较。采用酶联免疫吸附试验（ELISA）检测各组泪液和眼组织中MMP-9及MMP-9/TIMPs复合物的含量。结果：SM暴露组血清MMP-9水平显著高于对照组（P = 0.009）。SM暴露组眼部异常者血清MMP-9水平显著高于SM暴露组。有角膜钙化者血清MMP-9/TIMP-1水平显著高于无角膜钙化者（P = 0.045），有严重眼外伤者血清MMP-9/TIMP-1水平显著高于对照组（P = 0.046）。SM暴露组MMP-9/TIMP-4复合物水平显著低于对照组（P < 0.001）。SM暴露组中有泪膜和眼底异常者血清MMP-9/TIMP-4水平显著高于SM暴露组（P = 0.009和P = 0.020）。结论：SM暴露组中有眼部异常者血清MMP-9水平升高，而TIMP-1和TIMP-2水平无变化。SM暴露组血清TIMP-4水平明显降低，说明SM暴露对全身及眼部的损害程度较严重。
Introduction: Sulfur mustard (SM) intoxication produces local and systemic changes in the human body. In this study, the relationship between tear and serum matrix metalloproteinase (MMP)-9 and serum tissue inhibitors of metalloproteinases (TIMPs) are assessed in serious eye-injured SM-exposed casualties.Methods: A group of 128 SM-exposed patients with serious ocular injuries in three subgroups (19 mild, 31 moderate, and 78 severe cases) is compared with 31 healthy controls. Tear and ocular status and serum MMPs and MMP-9/TIMPs complex levels were evaluated using enzyme-linked immunosorbent assay (ELISA).Results: Serum level of MMP-9 was significantly higher in the SM-exposed group compared to the control group (P = 0.009). Mean serum MMP-9 level in the SM-exposed group with ocular abnormalities was significantly higher than that in the SM-exposed group without ocular abnormalities. SM-exposed people with corneal calcification had significantly higher serum MMP-9/TIMP-1 level compared to the SM-exposed ones without this problem (P = 0.045), The SM-exposed group with severe ocular injuries had significantly higher MMP-9/TIMP-1 than the controls (P = 0.046). The SM-exposed group had significantly lower levels of MMP-9/TIMP-4 complex than the controls (P < 0.001). The SM-exposed group with tear meniscus and fundus abnormality had significantly higher MMP-9/TIMP-4 levels than the SM-exposed group without these problems (P = 0.009 and P = 0.020).Conclusion: Serum MMP-9 level had increased in SM-exposed groups with ocular problems, while TIMP-1 and TIMP-2 levels had remained unchanged. Serum TIMP-4 drastically decreased in SM-exposed group, which clearly explains the severity of the systemic and ocular damages.