Rapid Raman Imaging of Stable, Functionalized Nanoshells in Mammalian Cell Cultures
Rapid Raman Imaging of Stable, Functionalized Nanoshells in Mammalian Cell Cultures
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DOI：
10.1021/nl901234x
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发表时间：
2009-08-01
期刊：
影响因子：
10.8
通讯作者：
Bishnoi, Sandra Whaley
中科院分区：
文献类型：
--
作者：
Huang, Yiming;Swarup, Vimal P.;Bishnoi, Sandra Whaley
两个拉曼活性聚（乙二醇）（PEG）分子，一个线性（MW 5000）和其他分支（MW 2420），合成稳定金-二氧化硅纳米壳在细胞培养基和跟踪纳米粒子在哺乳动物细胞培养。与市售PEG-硫醇或支链PEG相比，线性PEG在盐溶液中提供更大的纳米壳稳定性。表面增强的拉曼散射快速跟踪探针，并提供半定量信息的颗粒定位在小鼠巨噬细胞（RAW 264.7）和人乳腺癌（MCF 7）细胞培养。
Two Raman-active poly(ethylene glycol) (PEG) molecules, one linear (MW 5000) and the other branched (MW 2420), are synthesized to stabilize gold-silica nanoshells in cell culture media and track nanoparticles in mammalian cell cultures. The linear PEG provides greater nanoshell stability in saline solution compared to commercially available PEG-thiol or the branched PEG. Surface enhanced Raman scattering rapidly tracks the probes and provides semiquantitative information regarding particle localization within mouse macrophage (RAW 264.7) and human breast cancer (MCF 7) cell cultures.