QUANTITATIVE-ANALYSIS OF RIBOSOME BINDING-SITES IN ESCHERICHIA-COLI
QUANTITATIVE-ANALYSIS OF RIBOSOME BINDING-SITES IN ESCHERICHIA-COLI
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DOI：
10.1093/nar/22.7.1287
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发表时间：
1994-04-11
影响因子：
14.9
通讯作者：
STORMO, GD
中科院分区：
文献类型：
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作者：
BARRICK, D;VILLANUEBA, K;STORMO, GD
从-半乳糖苷酶编码区前的- 11位到0位，随机选取185个核糖体结合位点的克隆进行测序。测定每个无性系的翻译产量；它们相差超过3000倍。采用多元线性回归分析确定每个碱基在每个位置对翻译起始活性的贡献。已知对翻译起始很重要的特征，如起始密码子、Shine/Dalgarno序列、- 3位置碱基的身份和替代atg的发生，都被发现对活性有重要的定量影响。虽然二级结构的影响可以被视为异常值，但没有发现其他特征具有普遍意义。与大量天然大肠杆菌翻译起始位点的比较表明，尽管在数量上存在差异，但信息概况在质量上相似。这可能是由于自然集合中选择了较好的翻译起始位点，而随机集合的平均活性较低。
185 clones with randomized ribosome binding sites, from position - 11 to 0 preceding the coding region of beta-galactosidase, were selected and sequenced. The translational yield of each clone was determined; they varied by more than 3000-fold. Multiple linear regression analysis was used to determine the contribution to translation initiation activity of each base at each position. Features known to be important for translation initiation, such as the initiation codon, the Shine/Dalgarno sequence, the identity of the base at position - 3 and the occurrence of alternative ATGs, are all found to be important quantitatively for activity. No other features are found to be of general significance, although the effects of secondary structure can be seen as outliers. A comparison to a large number of natural E. coli translation initiation sites shows the information profile to be qualitatively similar although differing quantitatively. This is probably due to the selection for good translation initiation sites in the natural set compared to the low average activity of the randomized set.