Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana
Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana
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基于微阵列的拟南芥应激调节 microRNA 分析。
DOI：
10.1261/rna.895308
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发表时间：
2008-05-01
期刊：
影响因子：
4.5
通讯作者：
Zheng, Cheng-Chao
中科院分区：
文献类型：
--
作者：
Liu, Han-Hua;Tian, Xin;Zheng, Cheng-Chao
高盐度、干旱和低温是严重影响全球植物生长发育的三个常见环境胁迫因子。最近，microRNAs(MiRNAs)被认为是一类与应激反应相关的基因表达调控因子。然而，miRNA表达和应激反应之间的关系才刚刚开始探索。在这里，我们使用微阵列数据鉴定了14个胁迫诱导的miRNAs，在这些数据中，我们研究了三种非生物胁迫对拟南芥的影响。其中，分别检测到10个高盐、4个干旱和10个低温调节的miRNAs。MiR168、miR171和miR396对所有的压力都有反应。逆转录-聚合酶链式反应分析表明，高盐、干旱和低温胁迫信号通路之间存在很大的串扰。MiRNA启动子区域存在与胁迫相关的元件为我们的结果提供了进一步的证据。这些发现扩展了目前关于miRNA是压力条件下普遍存在的调节因子的观点。
High-salinity, drought, and low temperature are three common environmental stress factors that seriously influence plant growth and development worldwide. Recently, microRNAs (miRNAs) have emerged as a class of gene expression regulators that have also been linked to stress responses. However, the relationship between miRNA expression and stress responses is just beginning to be explored. Here, we identified 14 stress-inducible miRNAs using microarray data in which the effects of three abiotic stresses were surveyed in Arabidopsis thaliana. Among them, 10 high-salinity-, four drought-, and 10 cold-regulated miRNAs were detected, respectively. miR168, miR171, and miR396 responded to all of the stresses. Expression profiling by RT-PCR analysis showed great cross-talk among the high-salinity, drought, and cold stress signaling pathways. The existence of stress-related elements in miRNA promoter regions provided further evidence supporting our results. These findings extend the current view about miRNA as ubiquitous regulators under stress conditions.