Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated
Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated
复制标题
DOI：
10.1016/j.febslet.2004.05.047
复制
发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Pagès, JM
中科院分区：
文献类型：
--
作者：
Dupont, M;Dé, E;Pagès, JM
克隆了产气肠杆菌ompX基因。它的过度表达诱导的主要孔蛋白Omp36生产的减少，因此，β-内酰胺耐药被注意到。将纯化的外膜蛋白X（OmpX）重组到人工膜中，并形成在1 M NaCl中电导为20 pS且具有阳离子选择性的离子通道。MarA表达和高渗透压均诱导大肠杆菌中OmpX合成的显著增加。产气杆菌ATCC 13048菌株。此外，OmpX合成增加的条件下，表达的E。产气菌主要非特异性孔蛋白Omp36和Omp35减少。(C)2004年由Elsevier B.V.代表欧洲生物化学学会联合会出版。
The ompX gene of Enterobacter aerogenes was cloned. Its overexpression induced a decrease in the major porin Omp36 production and consequently a P-lactam resistance was noted. Purified outer membrane protein X (OmpX) was reconstituted into artificial membranes and formed ion channels with a conductance of 20 pS in 1 M NaCl and a cationic selectivity. Both MarA expression and high osmolarity induced a noticeable increase of the OmpX synthesis in the E. aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non-specific porins, Omp36 and Omp35, decreased. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.