Generation and Analysis of Chromosomal Contact Maps of Bacteria
Generation and Analysis of Chromosomal Contact Maps of Bacteria
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DOI：
10.1007/978-1-4939-7098-8_7
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Koszul, Romain
中科院分区：
文献类型：
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作者：
Marbouty, Martial;Koszul, Romain
本文描述了一种利用染色体构象捕获（3C）技术的全基因组衍生物生成细菌物种染色体接触图谱的方案。该方法很容易适用于各种各样的革兰刺和革兰氏细菌物种。它描述并说明了与该技术相关的已知警告和技术细节，任何有兴趣对其感兴趣的物种的基因组结构进行多尺度分析的实验室都应该感兴趣。
This methods article described a protocol aiming at generating chromosome contact maps of bacterial species using a genome-wide derivative of the chromosome conformation capture (3C) technique. The approach is readily applicable on a broad variety of gram thorn and gram-bacterial species. It describes and addresses known caveats and technicalities associated with the technique, and should be of interest to any laboratory interested to perform a multiscale analysis of the genome structure of its species of interest.