Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.)
Cadmium-tolerant plant growth-promoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern.)
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DOI：
10.1016/j.soilbio.2004.07.033
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发表时间：
2005-02-01
影响因子：
9.7
通讯作者：
Glick, BR
中科院分区：
文献类型：
--
作者：
Belimov, AA;Hontzeas, N;Glick, BR
从印度芥菜（Brassica juncea L.）Czern.）幼苗生长在镉补充土壤以及污水污泥和采矿废物严重污染镉。细菌对其他金属包括Zn、Cu、Ni和Co也表现出增加的耐受性。分离的菌株包括Variovorax paradoxus、Rhodococcus sp.和Flavobacterium sp.，并能促进B的根伸长。芥菜幼苗无论是在存在或不存在有毒镉浓度。一些菌株产生吲哚或铁载体，但没有一个具有C2 H2还原活性。除黄杆菌属菌株5 P-3外，所有菌株都含有1-氨基环丙烷-1-羧酸（ACC）脱氨酶，该酶将ACC（植物激素乙烯的直接前体）水解为NH 3和g.-酮丁酸盐矛盾弧菌利用ACC作为唯一的N或能量来源。ACC脱氨酶活性的细菌和它们的刺激作用根伸长之间的正相关性表明，利用ACC是一个重要的细菌性状，确定根生长促进。所分离的细菌提供了作为接种物以改善金属积累植物B的生长的希望。芥菜在有毒镉浓度的存在下，并为发展的plantinoculant系统，用于污染土壤的植物修复。(C)2004爱思唯尔有限公司保留所有权利。
Eleven cadmium-tolerant bacterial strains were isolated from the root zone of Indian mustard (Brassica juncea L. Czern.) seedlings grown in Cd-supplemented soils as well as sewage sludge and mining waste highly contaminated with Cd. The bacteria also showed increased tolerance to other metals including Zn, Cu, Ni and Co. The isolated strains included Variovorax paradoxus, Rhodococcus sp. and Flavobacterium sp., and were capable of stimulating root elongation of B. juncea seedlings either in the presence or absence of toxic Cd concentrations. Some of the strains produced indoles or siderophores, but none possessed C2H2-reduction activity. All the strains, except Flavobacterium sp. strain 5P-3, contained the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which hydrolyses ACC (the immediate precursor of plant hormone ethylene) to NH3 and g.-ketobutyrate. V. paradoxus utilized ACC as a sole source of N or energy. A positive correlation between the in vitro ACC deaminase activity of the bacteria and their stimulating effect on root elongation suggested that utilization of ACC is an important bacterial trait determining root growth promotion. The isolated bacteria offer promise as inoculants to improve growth of the metal accumulating plant B. juncea in the presence of toxic Cd concentrations and for the development of plantinoculant systems useful for phytoremediation of polluted soils. (C) 2004 Elsevier Ltd. All rights reserved.