Brefeldin A affects synthesis and integrity of a eukaryotic flagellum
Brefeldin A affects synthesis and integrity of a eukaryotic flagellum
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DOI：
10.1006/bbrc.1997.8015
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发表时间：
1998-01-26
影响因子：
3.1
通讯作者：
Fabry, S
中科院分区：
文献类型：
--
作者：
Haller, K;Fabry, S
真核生物的鞭毛和纤毛是高度动态的细胞器。在莱茵衣藻（Chlamydomonas reinhardtii）等绿藻中，鞭毛吸收和再合成是营养细胞周期的正常过程。快速再生也发生在应力诱导的鞭毛脱落后。Ca2+离子、蛋白质合成和激酶活性是已知影响再合成的主要因素。最近，我们已经检测到某些小G蛋白（Ypt/Rab）和GTPase调节剂（GDP解离抑制剂），被称为胞内囊泡运输的调节元件，存在于绿藻鞭毛膜中，这提高了细胞器的合成和/或完整性依赖于功能性膜运输的可能性。在这项研究中，我们研究了brefeldin A (BFA)，一种抑制动物和植物细胞内细胞膜流动和高尔基功能的抑制剂，对群体绿藻Gonium pectorale鞭毛再生的影响。我们发现低BFA浓度(
Eukaryotic flagella and cilia are highly dynamic organelles. In green algae like Chlamydomonas reinhardtii, flagella absorption and resynthesis is a normal process during the vegetative cell cycle. Rapid regeneration also occurs after stress-induced shedding of flagella. Ca2+ ions, protein synthesis, and a kinase activity are the main factors known to affect resynthesis. Recently, we have detected that certain small G proteins (Ypt/Rab) and a GTPase regulator (GDP dissociation inhibitor), known as regulatory elements of intracellular vesicle transport, are present in flagellar membranes of green algae, raising the possibility that the organelle's synthesis and/or integrity depends on functional membrane traffic. In this study, we examined the effect of brefeldin A (BFA), an inhibitor of intracellular membrane flow and Golgi function in animal and plant cells, on flagella regeneration in the colonial green alga Gonium pectorale. We show that low BFA concentrations (