Protection against Leptospira interrogans sensu lato challenge by DNA immunization with the gene encoding hemolysin-associated protein 1
Protection against Leptospira interrogans sensu lato challenge by DNA immunization with the gene encoding hemolysin-associated protein 1
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DOI：
10.1128/iai.73.7.4062-4069.2005
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
André-Fontaine, G
中科院分区：
文献类型：
--
作者：
Branger, C;Chatrenet, B;André-Fontaine, G
使用编码钩端螺旋体蛋白的DNA构建体是一种很有前景的钩端螺旋体病疫苗接种新方法。在之前的工作中，我们确定用腺病毒表达的溶血相关蛋白 1 (Hap1) (LipL32) 进行免疫可以对沙鼠的有毒钩端螺旋体攻击产生显着的保护作用。为了避免使用腺病毒载体，我们检查了 DNA 疫苗接种对致命攻击的临床保护作用。表达 Hap1 的 DNA 疫苗旨在增强该蛋白直接基因转移到沙鼠体内。在最后一次免疫后 3 周，用犬血清毒株进行攻击。我们的结果表明，Hap1 所共有的与钩端螺旋体致病菌株的交叉保护作用可以由 DNA 质粒载体介导。这一发现应该有助于针对细菌，特别是问号钩端螺旋体的新一代疫苗的设计和开发。
The use of DNA constructs encoding leptospiral proteins is a promising new approach for vaccination against leptospirosis. In previous work we determined that immunization with hemolysis-associated protein 1 (Hap1) (LipL32) expressed by adenovirus induced significant protection against a virulent Leptospira challenge in gerbils. To avoid the use of the adenovirus vector, we checked for clinical protection against lethal challenge by DNA vaccination. A DNA vaccine expressing Hap1 was designed to enhance the direct gene transfer of this protein into gerbils. A challenge was performed 3 weeks after the last immunization with a virulent strain of serovar canicola. Our results show that the cross-protective effect with pathogenic strains of Leptospira, shared by Hap1, could be mediated by the DNA plasmid vector. This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.