Measurement of macrophage growth and differentiation.
Measurement of macrophage growth and differentiation.
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DOI：
10.1002/0471142735.im1420s92
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发表时间：
2011-02
影响因子：
--
通讯作者：
Stanley, E Richard
中科院分区：
文献类型：
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作者：
Chitu, Violeta;Yeung, Yee-Guide;Yu, Wenfeng;Nandi, Sayan;Stanley, E Richard
通过直接细胞计数或DNA测量，该装置提供了测量琼脂培养物中巨噬细胞前体的丰度和生长以及体外分离的成熟巨噬细胞增殖的方案。本文还介绍了巨噬细胞的免疫组化鉴定方法和免疫荧光法测定巨噬细胞在体内的增殖状态。本文还介绍了通过免疫荧光分析细胞表面CSF-1受体表达来表征巨噬细胞分化的方法。
This unit provides protocols for measuring the abundance and growth of macrophage precursors in agar cultures and the proliferation of isolated mature macrophages in vitro, by either direct cell counting or by DNA measurement. Methods for the immunohistochemical identification of macrophages and the determination of their proliferative status in vivo by immunofluorescence are also included. It also describes methods for characterization of macrophage differentiation through the immunofluorescence analysis of cell surface expression of CSF-1 receptor.