Anthocyanin kinetics are dependent on anthocyanin structure
Anthocyanin kinetics are dependent on anthocyanin structure
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DOI：
10.1017/s000711451100314x
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Kurilich, Anne C.
中科院分区：
文献类型：
--
作者：
Novotny, Janet A.;Clevidence, Beverly A.;Kurilich, Anne C.
在人类饲养试验中研究了花青素代谢的动力学。志愿者（n 12）食用含有五种花青素形式的紫色胡萝卜：矢车菊素-3-（木糖-葡萄糖-半乳糖苷）、矢车菊素-3-（木糖-半乳糖苷）、矢车菊素-3-（木糖-芥子酰-葡萄糖-半乳糖苷）、矢车菊素-3-（木糖-阿魏酰-葡萄糖-半乳糖苷）和矢车菊素-3-（木糖-香豆酰-葡萄糖-半乳糖苷）。紫胡萝卜作为三种不同的治疗，在交叉设计与治疗之间的3周洗脱。紫胡萝卜处理为250 g生胡萝卜、250 g熟胡萝卜和500 g熟胡萝卜。在给药后8小时和24小时分别收集一系列血液和尿液样品，并分析花青素。在摄入的花青素形式中，在血浆和尿液中检测到四种：花青素-3-（木糖-葡萄糖-半乳糖苷）、花青素-3-（木糖-半乳糖苷）、花青素-3-（木糖-芥子酰-葡萄糖-半乳糖苷）和花青素-3-（木糖-阿魏酰-葡萄糖-半乳糖苷）。血浆和尿液中花青素含量的时间进程进行了评估与房室模型。结果表明，花青素的吸收、胃肠道转运和血浆消除依赖于花青素的结构。酰化的化合物（矢车菊素-3-（木糖-芥子酰-葡萄糖-半乳糖苷）和矢车菊素-3-（木糖-阿魏酰-葡萄糖-半乳糖苷））的吸收效率低于非酰化的花青素（矢车菊素-3-（木糖-葡萄糖-半乳糖苷）和矢车菊素-3-（木糖-半乳糖苷））。酰化花色苷的胃肠道吸收半衰期比非酰化花色苷短。非酰化化合物的分数消除比酰化花青素苷慢。这些结果提供了第一个信息的动力学的个人花青素在人类。
The kinetics of anthocyanin metabolism was investigated in a human feeding trial. Volunteers (n 12) consumed purple carrots containing five anthocyanin forms: cyanidin-3-(xylose-glucose-galactoside), cyanidin-3-(xylose-galactoside), cyanidin-3-(xylose-sinapoyl-glucose-galactoside), cyanidin-3-(xylose-feruloyl-glucose-galactoside) and cyanidin-3-(xylose-coumuroyl-glucose-galactoside). The purple carrots were served as three different treatments in a crossover design with a 3-week washout between treatments. Purple carrot treatments were 250 g raw carrots, 250 g cooked carrots and 500 g cooked carrots. Serial blood and urine samples were collected for 8 and 24 h after the dose, respectively, and analysed for anthocyanins. Of the anthocyanin forms ingested, four were detected in plasma and urine: cyanidin-3-(xylose-glucose-galactoside), cyanidin-3-(xylose-galactoside), cyanidin-3-(xylose-sinapoyl-glucose-galactoside) and cyanidin-3-(xylose-feruloyl-glucose-galactoside). The time courses of plasma and urine anthocyanin contents were evaluated with compartmental modelling. Results showed that absorption, gastrointestinal transit and plasma elimination are dependent on anthocyanin structure. Absorption efficiencies of acylated compounds (cyanidin-3-(xylose-sinapoyl-glucose-galactoside) and cyanidin-3-(xylose-feruloyl-glucose-galactoside)) were less than those for non-acylated anthocyanins (cyanidin-3-(xylose-glucose-galactoside) and cyanidin-3-(xylose-galactoside)). The acylated anthocyanins exhibited a shorter half-life for gastrointestinal absorption than the non-acylated anthocyanins. Fractional elimination of non-acylated compounds was slower than that for acylated anthocyanins. These results provide the first information about the kinetics of individual anthocyanins in human beings.