Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.
Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.
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DOI：
10.1186/s13568-015-0127-2
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发表时间：
2015-12
期刊：
影响因子：
3.7
通讯作者：
Ueda M
中科院分区：
文献类型：
--
作者：
Kitahara N;Morisaka H;Aoki W;Takeda Y;Shibasaki S;Kuroda K;Ueda M
白色念珠菌是一种条件致病菌，可导致免疫功能低下的宿主发生致命性疾病。寄主对C.白色念珠菌依赖于巨噬细胞对病原体的摄取。对C.白念珠菌从巨噬细胞是需要了解系统性念珠菌病的发病。在这项研究中，自然的相互作用的C。采用高效硅胶毛细管整体柱对白念珠菌和巨噬细胞进行蛋白质组分析。利用该系统，我们发展了一种“混合定量蛋白质组分析”方法。通过nanoLC-MS/MS同时分析白色念珠菌和巨噬细胞，而不需要分离两个单独的活细胞。来自C的227种蛋白质。白念珠菌和五种来自巨噬细胞的蛋白质被鉴定为候选的相互作用特异性分子。C.白念珠菌似乎通过β-氧化途径、乙醛酸循环和代谢生成产生葡萄糖以逃避巨噬细胞。C.胁迫相关蛋白和候选致病蛋白的上调。albicans表明C.白色念珠菌忍受了巨噬细胞内的恶劣环境。巨噬细胞内糖代谢相关蛋白NOA 1和分子伴侣HSPA 1A合成的下调表明C.白色念珠菌能够部分地通过抑制这些巨噬细胞蛋白的产生而从巨噬细胞逃逸。本文的在线版本（doi：10.1186/s13568-015-0127-2）包含补充材料，可供授权用户使用。
Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand the onset of systemic candidiasis. In this study, native interactions of C. albicans with macrophages were investigated by proteome analysis using high efficiency of long monolithic silica capillary column. Using this system, we developed a method of “mixed and quantitative proteome analysis” in which C. albicans and macrophages were simultaneously analyzed by nanoLC–MS/MS without the need to isolate the two individual living cells. Two hundred twenty-seven proteins from C. albicans and five proteins from macrophages were identified as candidate interaction-specific molecules. C. albicans seemed to produce glucose through a β-oxidation pathway, a glyoxylate cycle, and gluconeogenesis for escape from macrophages. Up-regulation of stress-related and candidate pathogenic proteins in C. albicans indicated how C. albicans endured the harsh environment inside the macrophages. Down-regulation of apoptosis-associated protein NOA1- and chaperone HSPA1A-syntheses in macrophage indicated that C. albicans was able to escape from macrophages in part by suppressing the production of these macrophage proteins. The online version of this article (doi:10.1186/s13568-015-0127-2) contains supplementary material, which is available to authorized users.