WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis
WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis
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DOI：
10.1111/j.1365-313x.2004.02235.x
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发表时间：
2004-11-01
期刊：
影响因子：
7.2
通讯作者：
Benning, C
中科院分区：
文献类型：
--
作者：
Cernac, A;Benning, C
种子发育过程中贮藏化合物的积累确保了幼苗的存活，并以食物和饲料的形式为人类和动物提供营养。AP2/EREBP转录因子WRINKLED1（WRI1）参与拟南芥种子贮藏代谢的调控。一个剪接突变体等位基因wri1 - 1导致种子油积累减少。糖酵解在这个突变体中受到损害，使得发育中的胚胎不能有效地将蔗糖转化为三酰甘油生物合成的前体。在花椰菜花叶病毒35S启动子的控制下表达WRINKLED1 cDNA导致种子含油量增加。此外，WRINKLED1 cDNA的异位表达引起了三酰甘油在发育中的幼苗的积累。这种效应取决于生长培养基中葡萄糖的存在或其他容易代谢为葡萄糖的糖。油积累幼苗表现出异常的发展与延长胚胎状态相一致。
The accumulation of storage compounds during seed development ensures the survival of the young seedling, and also provides nutrition to humans and animals in the form of foods and feeds. The putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis. A splicing mutant allele, wri1-1, caused the reduction of seed oil accumulation. Glycolysis was compromised in this mutant, rendering developing embryos unable to efficiently convert sucrose into precursors of triacylglycerol biosynthesis. Expression of the WRINKLED1 cDNA under the control of the cauliflower mosaic virus 35S-promoter led to increased seed oil content. Moreover, the ectopic expression of the WRINKLED1 cDNA caused the accumulation of triacylglycerols in developing seedlings. This effect depended upon the presence of glucose in the growth medium or other sugars readily metabolized to glucose. Oil-accumulating seedlings showed aberrant development consistent with a prolonged embryonic state.