Expression of neurotransmitters, vasculogenesis markers and myosin heavy chain isoforms in the masseter muscle of senescence‐accelerated mouse prone 8 mice
Expression of neurotransmitters, vasculogenesis markers and myosin heavy chain isoforms in the masseter muscle of senescence‐accelerated mouse prone 8 mice
复制标题
衰老加速小鼠俯卧8只小鼠咬肌中神经递质、血管生成标记物和肌球蛋白重链亚型的表达
DOI：
10.1111/joor.13449
复制
发表时间：
2023
影响因子：
2.9
通讯作者：
Itoh Masahiro
中科院分区：
文献类型：
--
作者：
Nakamura Chiaki;Sato Iwao;Ueda Yoko;Kawata Shinichi;Nagahori Kenta;Omotehara Takuya;Yakura Tomiko;Natsuyama Yutaro;Li Zhong‐Lian;Itoh Masahiro
无牙和软饮食喂养引起的海马区学习记忆障碍和病理改变与咬肌功能降低有关。目的不同咀嚼条件下咬肌中肌球蛋白重链(MyHC)亚型的表达也会发生变化。神经递质降钙素基因相关肽(CGRP)和血管内皮生长因子A(VEGF-A)参与多发性骨髓瘤的形成。方法采用实时定量聚合酶链式反应和原位杂交的方法，研究了衰老加速小鼠MMS中降钙素基因相关蛋白、血管内皮生长因子-A和MyHC亚型基因表达的变化。结果定量实时聚合酶链式反应显示SIMP小鼠MM(P<； .001)中降钙素基因相关蛋白水平明显升高。24周龄SAMP8小鼠MM中MyHC-iid/x基因表达高于24周龄SAMR1小鼠(p<； .001)，而慢MyHC SAMP8小鼠低于SAMR1小鼠(p<； .001)。通过原位杂交观察到SAMP8小鼠MM的肌纤维上有CGRP的表达，而SAMR1小鼠MM的肌肉纤维上没有CGRP的表达。主成分分析显示，12周和24 时，SAMP8-MyHC-iid/x、SAMP8-CGRP、SAMR1-MyHC-emb、SAMR1-CGRP、SAMR1-VEGFA、SAMR1-CD31、SAMP8-VEGFA和SAMP8-CD31在MM中均有较强的阳性贡献。
BackgroundLearning and memory deficits and pathologic changes in the hippocampus caused by toothlessness and soft diet feeding are related to reduced masseter muscle (MM) function.ObjectiveMyosin heavy chain (MyHC) isoform expression in the MM also changes under different chewing conditions. The neurotransmitter calcitonin gene‐related peptide (CGRP) and vascular endothelial growth factor A (VEGF‐A) are involved in MM formation. However, the relationship between CGRP, VEGF‐A and MyHC isoforms in the MM in the senescence‐accelerated mouse prone 8 (SAMP8) strain, a model of learning and memory deficits, remains unclear.MethodsChanges in CGRP, VEGF‐A, vasculogenesis marker and MyHC isoform mRNA expression in the MMs of ageing SAMP8 and senescence‐accelerated mouse resistant 1 (SAMR1) mice was investigated through quantitative real‐time polymerase chain reaction (qRT–PCR) andin situhybridization.ResultsqRT–PCR revealed obviously high CGRP levels in the SAMP8 mouse MM (p< .001). MyHC‐IId/x mRNA expression in the MM was higher in 24‐week‐old SAMP8 mice than 24‐week‐old SAMR1 mice (p< .001) but lower in slow‐MyHC SAMP8 mice than SAMR1 mice (p< .001). CGRP mRNA was observed on the muscle fibres of the SAMP8 mouse MM but not the SAMR1 mouse MM throughin situhybridization. Principal component analysis (PCA) revealed strong positive contributions of SAMP8‐MyHC‐IId/x, SAMP8‐CGRP, SAMR1‐MyHC‐emb, SAMR1‐CGRP, SAMR1‐VEGF‐A, SAMR1‐CD31, SAMP8‐VEGF‐A, and SAMP8‐CD31 in the MM at 12 and 24 weeks.ConclusionCalcitonin gene‐related peptide is also key for the MyHC‐IId/x and slow‐MyHC patterns in the MMs of SAMP8 mice.