Clinical and microbiological features of refractory periodontitis subjects
Clinical and microbiological features of refractory periodontitis subjects
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DOI：
10.1111/j.1600-051x.1998.tb02424.x
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发表时间：
1998-02-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
--
作者：
Colombo, AP;Haffajee, AD;Socransky, SS
本研究的目的是比较成功治疗和难治性牙周炎患者的临床参数和40种龈下菌种的患病率和水平。94名受试者接受了牙周刮治和牙根刨平，如有需要，还接受了牙周手术和系统四环素治疗。治疗后一年内，28名难治性受试者出现全口平均附着丧失和/或bbb30, 3个部位出现附着丧失>2.5 mm， 66名成功治疗的受试者显示平均附着水平增加，没有任何部位出现附着丧失>2.5 mm。采用全基因组DNA探针和棋盘格DNA-DNA杂交技术，从每颗牙齿的中侧面采集基线龈下菌斑样本，确定40个龈下分类群的存在和水平。计算每个受试者的平均水平和每个物种的定殖点百分比（患病率），并使用Mann-Whitney检验寻求组间差异。在所检测的40种细菌中，包括放线菌、牙龈卟啉单胞菌、牙齿密螺旋体和连翘拟杆菌在内的大多数细菌在难治性组中发病率相等或较低。黑化普雷沃氏菌在治疗成功的患者中更为普遍，而难治性患者中链球菌种类的比例更大，尤其是星座链球菌。受试者难治性的几率为8.6 (p
The purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year post-therapy 66 successfully treated subjects showed mean attachment level gain and no sites with attachment loss >2.5 mm. Baseline subgingival plaque samples were taken from the mesial aspect of each tooth and the presence and levels of 40 subgingival taxa were determined using whole genomic DNA probes and checkerboard DNA-DNA hybridization. The mean levels and % of sites colonized by each species (prevalence) was computed for each subject and differences between groups sought using the Mann-Whitney test. Most of the 40 species tested, including Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola and Bacteroides forsythus, were equally or less prevalent in the refractory group. Prevotella nigrescens was significantly more prevalent in successfully treated subjects, while refractory subjects harbored a larger proportion of Streptococcus species, particularly Streptococcus constellatus. The odds of a subject being refractory was 8.6 (p