INDUCTION OF A MESENCEPHALIC PHENOTYPE IN THE 2-DAY-OLD CHICK PROSENCEPHALON IS PRECEDED BY THE EARLY EXPRESSION OF THE HOMEOBOX GENE EN
INDUCTION OF A MESENCEPHALIC PHENOTYPE IN THE 2-DAY-OLD CHICK PROSENCEPHALON IS PRECEDED BY THE EARLY EXPRESSION OF THE HOMEOBOX GENE EN
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DOI：
10.1016/0896-6273(91)90237-t
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发表时间：
1991-06-01
期刊：
影响因子：
16.2
通讯作者：
ALVARADOMALLART, RM
中科院分区：
文献类型：
--
作者：
MARTINEZ, S;WASSEF, M;ALVARADOMALLART, RM
同源盒基因en与果蝇基因同源，在脊椎动物神经管的后脑-中脑段表达。利用鹌鹑-鸡嵌合体、一种抗en蛋白抗体和细胞结构技术，我们证明，在E2时，后脑移植到前脑，维持了高水平的en蛋白和其假定的小脑命运。异位中脑在相邻寄主前脑中诱导en的表达，随后产生中脑表型。这些相关的遗传和表型表达表明，转录调控基因en参与了小脑和中脑细胞分化。en在鸡前脑中也能被哺乳动物后脑移植诱导表达，表明调控en转录的因子在系统发育上具有良好的保守性。
The homeobox gene en, homologous to the gene engrailed of Drosophila, is expressed in the metencephalic-mesencephalic segment of the vertebrate neural tube. Using quail-chick chimeras, an antibody against en proteins, and cytoarchitectonic techniques, we demonstrate that metencephalon transplanted to prosencephalon, at E2, maintains a high level of en proteins and its presumptive cerebellar fate. The ectopic metencephalon induces in the contiguous host prosencephalon the expression of en and, subsequently, a mesencephalic phenotype. These related genetic and phenotypic expressions indicate that the transcriptional regulatory en gene is involved in cerebellar and mesencephalic cytodifferentiation. The expression of en can also be induced in chick prosencephalon by a mammalian metencephalic graft, indicating that the factors regulating the transcription of en are phylogenetically well conserved.