Temperature-dependent development and the significance for estimating postmortem interval of Chrysomya nigripes Aubertin, a new forensically important species in China
Temperature-dependent development and the significance for estimating postmortem interval of Chrysomya nigripes Aubertin, a new forensically important species in China
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DOI：
10.1007/s00414-016-1315-6
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发表时间：
2016-09-01
影响因子：
2.1
通讯作者：
Lai, Yue
中科院分区：
文献类型：
--
作者：
Li, Liangliang;Wang, Yu;Lai, Yue
在法医昆虫学中，肉食性蝇的发育持续时间和幼虫体长是目前估计最小死后间隔（PMImin）的两种主要方法。尸体上的主要物种丽丽科 (Calliphoridae) 的发育阶段的完整特征对于 PMI 估算尤其重要。 Chrysomya nigripes Aubertin主要在腐烂和腐烂后阶段出现在尸体上，对于PMImin估计具有重要价值。然而，关于此类物种发展的详细报告有限。因此，本研究旨在探讨黑腹鳅的发育规律，为PMI估算提供基础数据。在这项研究中，从放置在田间的猪尸体中收集了黑腹黑线虫的幼虫。将菌落在 16、20、24、28 和 32 (+/- 1) A 摄氏度的恒定温度下培养。研究了不同温度下 C. nigripes 的生物学特征、发育模式和体长随时间的变化。结果表明，20℃、24℃、28℃和32℃（+/-1）℃下黑斑鳅幼虫的平均发育持续时间分别为608.0+/-68.0h、327.0+/-53.8h、254.0+/-36.5h和217.0+/-28.0h。体长随发育时间的增长分为两个阶段：生长期和平台期。最大体长为11-12mm，体长与发育时间的关系可以用以下方程模拟：L=a+bT+cT(2)+dT(3)。未获得 16 A 摄氏度下的发育持续时间和幼虫体长的数据。综上所述，本研究全面研究了C. nigripes的发育生物学，对于估算高度腐烂尸体的PMI具有重要价值。
In forensic entomology, the developmental duration and larval body length of sarcosaprophagous flies are presently the two major approaches to estimate minimal postmortem interval (PMImin). A full characterization of the developmental stages of Calliphoridae, a predominating species on corpses, is especially important for PMI estimation. Chrysomya nigripes Aubertin, which appears on the corpse mainly during the decay and the post-decay stages, is of significant value for PMImin estimation. However, there are limited detailed reports on the development of such species. Hence, this study was conducted to address the development pattern of C. nigripes, in order to provide the basic data related to PMI estimation. In this study, the larvae of C. nigripes were collected from pig carcasses that were placed in the field. The colonies were cultured at constant temperatures of 16, 20, 24, 28, and 32 (+/- 1) A degrees C. The biological features of C. nigripes, the developmental pattern, and the body length with time at different temperatures were studied. The results showed that the average developmental duration of the C. nigripes larvae at 20, 24, 28, and 32 (+/- 1) A degrees C were 608.0 +/- 68.0 h, 327.0 +/- 53.8 h, 254.0 +/- 36.5 h, and 217.0 +/- 28.0 h, respectively. There were two phases of body length increment with developmental time: growing phase and plateau phase. The maximal body length was 11-12 mm, and the relationship between body length and developmental time can be simulated using the following equation: L = a + bT + cT(2) + dT(3). The data for developmental duration and larval body length at 16 A degrees C were not obtained. In summary, this study comprehensively studied the developmental biology of C. nigripes, which has a significant value for estimating PMI from highly decayed carcasses.