Genome sequences of two Salmonella enterica strains (MEZSAL74 and MEZSAL81) harbouring multiple antimicrobial resistance genes isolated from livestock in South Africa.
Genome sequences of two Salmonella enterica strains (MEZSAL74 and MEZSAL81) harbouring multiple antimicrobial resistance genes isolated from livestock in South Africa.
复制标题
从南非牲畜中分离出的两种肠道沙门氏菌菌株（MEZSAL74 和 MEZSAL81）的基因组序列，其中含有多种抗菌素耐药性基因。
DOI：
10.1016/j.jgar.2020.04.001
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发表时间：
2020
影响因子：
4.6
通讯作者：
Järhult,JosefD
中科院分区：
文献类型：
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作者：
ElZowalaty,MohamedE;Hickman,RachelA;Mthembu,ThobekaP;Zishiri,OliverT;ElZowalaty,AhmedE;Järhult,JosefD
家畜沙门氏菌肠道感染对全球公共卫生构成严重威胁。在这里，我们首次报告了两个多重耐药的家畜相关的基因组序列草图。从南非的一只鸡和一头牛中分离出一株沙门氏菌。使用Illumina MiSeq平台对肠杆菌菌株MEZSAL 74和MEZSAL 81进行测序。将生成的读段修剪并从头组装。分析组装的重叠群的抗菌素耐药基因、染色体突变和染色体外质粒。还进行了多位点序列分型（MLST）。为了将MEZSAL 74和MEZSAL 81与其它已测序的S. entericasolates，原始读取序列被下载，所有的序列文件进行了相同的处理，以产生一个自举的最大似然系统发育树。ResultsExtrachromosomal质粒和抗菌药物耐药性的遗传决定因素检测在两个测序的细菌分离株氨基糖苷类和氟喹诺酮类。经MLST分析，MEZSAL 74属于未知序列型（ST），MEZSAL 81属于ST 33。结论获得的MEZSAL 74和MEZSAL 81的基因组序列可为家畜相关耐药菌的分子流行病学研究提供参考。进入非洲。
ObjectivesAntimicrobial-resistant livestock-associatedSalmonella entericainfections pose a significant public-health threat worldwide. Here we report for the first time the draft genome sequences of two multidrug-resistant livestock-associatedS. entericastrains isolated from a chicken and a cow in South Africa.MethodsGenomic DNA ofS. entericastrains MEZSAL74 and MEZSAL81 was sequenced using an Illumina MiSeq platform. The generated reads were trimmed and de novo assembled. The assembled contigs were analysed for antimicrobial resistance genes, chromosomal mutations and extrachromosomal plasmids. Multilocus sequence typing (MLST) was also performed. In order to compare isolates MEZSAL74 and MEZSAL81 with other previously sequencedS. entericaisolates, raw read sequences were downloaded and all sequence files were treated identically to generate a bootstrapped maximum likelihood phylogenetic tree.ResultsExtrachromosomal plasmids and genetic determinants of antimicrobial resistance were detected in both sequenced bacterial isolates to aminoglycosides and fluoroquinolones. By MLST, strain MEZSAL74 belonged to an unknown sequence type (ST) and strain MEZSAL81 belonged to ST33.ConclusionThe genome sequences of strains MEZSAL74 and MEZSAL81 reported here will serve as a reference for molecular epidemiological studies of antimicrobial-resistant livestock-associatedS. entericain Africa.