Transient transfection of oligodendrocyte progenitors by electroporation
Transient transfection of oligodendrocyte progenitors by electroporation
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DOI：
10.1023/a:1022426021173
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发表时间：
1998-03-01
影响因子：
4.4
通讯作者：
Pfeiffer, SE
中科院分区：
文献类型：
--
作者：
Krueger, WHH;Madison, DL;Pfeiffer, SE
将转基因瞬时转染到少突胶质细胞中，为研究髓鞘形成过程中蛋白质的功能提供了重要的工具。然而，目前建立的程序通常导致低存活率和低水平的转基因摄取到初级少突胶质细胞祖细胞。我们描述了一种电穿孔方法，该方法产生瞬时转染少突胶质细胞祖细胞，可产生高达10-15%的存活细胞，并且每次电穿孔反应可提供大约10(4)个存活的转染细胞。在最近的应用中，随着转染的祖细胞在少突胶质细胞谱系的后续阶段的进展，转基因表达持续存在。该技术有望促进对原代培养少突胶质细胞发育过程中关键蛋白和脂质功能的研究。
The transient transfection of transgenes into oligodendrocytes offers an important tool for studying the function of proteins during myelin formation. Currently established procedures, however, have generally resulted in low survival rates and low levels of uptake of the transgene into primary oligodendrocyte progenitors. We describe an electroporation method which yields transient transfection of oligodendrocyte progenitors of up to 10-15% of the surviving cells, and provides approximately 10(4) surviving, transfected cells per electroporation reaction. In recent applications transgene expression persisted as the transfected progenitors progressed through subsequent stages of the oligodendrocyte lineage. This technique is expected to facilitate the study of the function of key proteins and lipids during the development of primary cultured oligodendrocytes.