A new hepatitis B virus variant in a chronic carrier with multiple episodes of viral reactivation and acute hepatitis.
A new hepatitis B virus variant in a chronic carrier with multiple episodes of viral reactivation and acute hepatitis.
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DOI：
10.1016/0042-6822(90)90274-u
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发表时间：
1990-11
期刊：
影响因子：
3.7
通讯作者：
G. Raimondo;Ralf Schneider;M. Stemler;V. Smedile;G. Rodinò;H. Will
中科院分区：
文献类型：
--
作者：
G. Raimondo;Ralf Schneider;M. Stemler;V. Smedile;G. Rodinò;H. Will
为了检查是否有尚未描述的由于前C区突变而不能表达HBe的乙肝病毒变异，以及如果存在，它们是否导致特定的感染和疾病过程，我们分析了一名HBs/抗-HBe阳性慢性携带者的HBV基因组，该携带者有几次慢性肝炎急性再加重，至少有两个病毒期。对聚合酶链式反应扩增的两个病毒期的前C/C区和前S区序列进行直接测序，发现它们彼此非常相似，但与已发表的乙肝病毒基因组有很大的差异。这两个病毒群体都包含前C翻译起始密码子(AUG/CUG)的第一个核苷酸突变，该突变阻止了HBeAg的表达。这些数据表明，存在一种新的乙肝病毒变异株，该变异株可进入潜伏期，在肝脏炎症急性加重时可从潜伏期重新激活。
To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexacerbations of chronic hepatitis with at least two viremic phases. Direct sequencing of the precore/core and the pre-S regions of the HBV sequences of both viremic phases amplified by the polymerase chain reaction revealed that they were very similar to each other but substantially divergent from published HBV genomes. Both virus populations contained a mutation in the first nucleotide of the pre-C translation initiation codon (AUG/CUG) which prevents HBeAg expression. These data demonstrate the existence of a new HBV variant which can enter a latent phase from which it can be reactivated with acute reexacerbation of liver inflammation.