Isolation of amniotic stem cell lines with potential for therapy
Isolation of amniotic stem cell lines with potential for therapy
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DOI：
10.1038/nbt1274
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发表时间：
2007-01-01
影响因子：
46.9
通讯作者：
Atala, Anthony
中科院分区：
文献类型：
--
作者：
De Coppi, Paolo;Bartsch, Georg, Jr.;Atala, Anthony
能够分化成多种谱系的干细胞可能对治疗有价值。我们报告分离人类和啮齿动物羊水来源的干细胞（AFS）表达胚胎和成人干细胞标记。未分化的AFS细胞在没有饲养细胞的情况下广泛扩增，在36小时内加倍，并且不具有致瘤性。保持超过250个种群的品系保留了长端粒和正常核型。AFS细胞具有广泛的多能性。通过逆转录病毒标记验证的克隆人系被诱导分化成代表每个胚胎胚层的细胞类型，包括成脂细胞、成骨细胞、成肌细胞、内皮细胞、神经细胞和肝细胞系。来源于人AFS细胞并显示特殊功能的分化细胞的实例包括分泌神经递质L-谷氨酸或表达G-蛋白门控内向整流钾通道的神经元谱系细胞、产生尿素的肝谱系细胞和形成组织工程化骨的成骨谱系细胞。
Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.