Brain ankyrin. A membrane-associated protein with binding sites for spectrin, tubulin, and the cytoplasmic domain of the erythrocyte anion channel.
Brain ankyrin. A membrane-associated protein with binding sites for spectrin, tubulin, and the cytoplasmic domain of the erythrocyte anion channel.
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发表时间：
1984-11
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通讯作者：
J. Davis;V. Bennett
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作者：
J. Davis;V. Bennett
脑锚蛋白纯化从猪脑细胞膜毫克数量的程序，涉及亲和层析红细胞spectrinagarose。脑锚蛋白包括Mr = 210，000和220，000的两种多肽，它们通过肽图谱几乎相同，并且在溶液中是单体。脑锚蛋白和红细胞锚蛋白是密切相关的蛋白质，具有以下共同特性：1）共有抗原位点，2）在血影蛋白四聚体的中间区域与血影蛋白β亚基的高亲和力结合，3）红细胞阴离子通道的胞质结构域的结合位点，4）微管蛋白的结合位点，5）与Mr = 72的蛋白酶抗性结构域相似的结构域结构，000，其含有血影蛋白结合活性和Mr = 95，000（脑锚蛋白）或90，000（红细胞锚蛋白）的结构域，其含有微管蛋白和阴离子通道的结合位点。基于放射免疫测定，脑锚蛋白以约100 pmol/mg膜蛋白存在于脱髓鞘膜中，所述放射免疫测定使用针对脑锚蛋白产生的抗体并在脑锚蛋白-琼脂糖上亲和纯化。脑血影蛋白四聚体以30 pmol/mg膜蛋白存在。因此，脑锚蛋白以足够的量存在，以将血影蛋白附着到膜上。脑锚蛋白也可以独立于血影蛋白将微管附着到膜上，并且具有将微管和血影蛋白相关的肌动蛋白丝相互连接的潜力。
Brain ankyrin was purified from pig brain membranes in milligram quantities by a procedure involving affinity chromatography on erythrocyte spectrinagarose. Brain ankyrin included two polypeptides of Mr = 210,000 and 220,000 that were nearly identical by peptide mapping and were monomers in solution. Brain ankyrin and erythrocyte ankyrin are closely related proteins with the following properties in common: 1) shared antigenic sites, 2) high-affinity binding to the spectrin beta subunit at the midregion of spectrin tetramers, 3) a binding site for the cytoplasmic domain of the erythrocyte anion channel, 4) a binding site for tubulin, 5) a similar domain structure with a protease-resistant domain of Mr = 72,000 that contains the spectrin-binding activity and domains of Mr = 95,000 (brain ankyrin) or 90,000 (erythrocyte ankyrin) that contain binding sites for both tubulin and the anion channel. Brain ankyrin is present at about 100 pmol/mg of membrane protein in demyelinated membranes based on radioimmunoassay with antibody raised against brain ankyrin and affinity purified on brain ankyrin-agarose. Brain spectrin tetramers are present at 30 pmol/mg of membrane protein. Brain ankyrin thus is present in sufficient amounts to attach spectrin to membranes. Brain ankyrin also may attach microtubules to membranes independently of spectrin and has the potential to interconnect microtubules and spectrin-associated actin filaments.