Bioactive cochlear implant electrodes with functionalised calcium phosphate nanoparticles
Bioactive cochlear implant electrodes with functionalised calcium phosphate nanoparticles
复制标题
具有功能化磷酸钙纳米颗粒的生物活性人工耳蜗电极
DOI：
10.1055/s-0039-1686400
复制
发表时间：
2019
影响因子：
1
通讯作者：
S. Hansen
中科院分区：
文献类型：
--
作者：
L. Holtmann;K. Wey;R. Schirrmann;S. Brandau;M. Epple;S. Lang;S. Hansen
方法：为了解决这个问题，DNA负载的CaP-NP被镀在耳蜗电极上，并通过扫描电子显微镜和X射线光谱法进行表征。其次，以与聚赖氨酸结合的逐层方法用CaP-NP包被玻璃盖玻片。结果：在CI电极上电泳是可行的。然而，只能附着少量浓度的CaP-NP。相反，逐层方法允许更高浓度的CaP-NP沉淀在表面上。内耳细胞的免疫组织化学染色显示足够的生物相容性的CaP-NP，包括非神经细胞增殖和轴突发芽的内耳cells.Discussion：涂层表面与功能化的纳米粒子是一种可行的和成功的方法。逐层方法使得能够铺板更高量的CaP-NP，从而更有效地运输蛋白质、DNA、RNA或药物。CaP-NP对内耳细胞显示出足够的生物相容性。
Methods:To address this issue, DNA-loaded CaP-NP were plated on cochlea electrodes and characterised by scanning electron microscopy and x-ray-spectrometry. Secondly, glass cover slips were coated with CaP-NP in a layer-by-layer method combined with polylysine. Interaction between CaP-NP and inner ear cells was analyzed via immunhistochemical staining of inner ear cells of newborn rats.Results:Electrophoretic plating on CI-electrodes was feasible. However, only minor concentrations of CaP-NP could be attached. In contrast, a layer-by-layer method allowed higher concentrations of CaP-NP to be precipitated on the surface. Immunhistochemical staining of the inner ear cells revealed adequate biocompatibility of CaP-NP including non-neural cell proliferation and axonal sprouting of inner ear cells.Discussion:Coating surfaces with functionalised nanoparticles is a feasible and successful method. A layer-by-layer method enables plating of higher amounts of CaP-NP and thus more efficient transport of proteins, DNA, RNA, or drugs. CaP-NP show sufficient biocompatibility towards inner ear cells.