Neutrophil apoptosis in the acute respiratory distress syndrome
Neutrophil apoptosis in the acute respiratory distress syndrome
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DOI：
10.1164/ajrccm.156.6.96-12081
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发表时间：
1997-12-01
影响因子：
24.7
通讯作者：
Martin, TR
中科院分区：
文献类型：
--
作者：
MatuteBello, G;Liles, WC;Martin, TR
中性粒细胞（PMN）凋亡在急性呼吸窘迫综合征（ARDS）中的作用尚不清楚。我们采用形态学标准对35例患者在ARDS第1、3、7、14和21天的支气管肺泡灌洗液（BAL）中凋亡的PMN进行计数，并对13例患者在ARDS风险第1和3天的BALF中凋亡的PMN进行计数。我们发现在整个ARDS过程中，BAL中凋亡的PMN比例很低。在高危患者和已确诊的ARDS患者中，以及存活患者（2.4%）和死亡患者（1.8%）之间，凋亡的PMN百分比没有显著差异。当正常人PMN在ARDS BAL中孵育时，与在正常志愿者灌洗液中孵育的PMN（76 +/-7%，p < 0.05）相比，凋亡的比例明显较低（50 +/-4%）。G-CSF和GM-CSF的免疫耗竭BAL可阻断ARDS BAL的这种抗凋亡作用。我们的结论是，在整个ARDS过程中，从ARDS患者肺中恢复的凋亡中性粒细胞的比例是低的。此外，在体外，来自ARDS患者的BAL降低了正常人PMN的存活，并且这种效应部分由C-CSF和GM-CSF介导。
Little is known about neutrophil (PMN) apoptosis in the acute respiratory distress syndrome (ARDS). We used morphologic criteria to count apoptotic PMN in bronchoalveolar ravage fluid (BAL) of 35 patients on Days 1, 3, 7, 14, and 21 of ARDS and 13 patients on Days 1 and 3 of risk for ARDS. We found that the proportion of apoptotic PMN in BAL was low throughout the course of ARDS. There was no significant difference between the percentage of apoptotic PMN in patients at risk and patients with established ARDS or between patients who lived (2.4%) and patients who died (1.8%). When normal human PMN were incubated in ARDS BAL, a significantly lower proportion became apoptotic (50 +/- 4%), as compared with PMN incubated in lavage fluid from normal volunteers (76 +/- 7%, p < 0.05). This antiapoptotic effect of ARDS BAL was blocked by immunodepleting BAL of G-CSF and GM-CSF. We conclude that the proportion of apoptotic PMN recovered from the lungs of patients with ARDS is low throughout the course of ARDS. Furthermore, BAL from patients with ARDS prolongs survival of normal human PMN in vitro, and this effect is partially mediated by C-CSF and GM-CSF.