Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini-infected Syrian golden hamsters.
Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini-infected Syrian golden hamsters.
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发表时间：
1978-12
期刊：
影响因子：
11.2
通讯作者：
W. Thamavit;N. Bhamarapravati;S. Sahaphong;S. Vajrasthira;S. Angsubhakorn
中科院分区：
文献类型：
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作者：
W. Thamavit;N. Bhamarapravati;S. Sahaphong;S. Vajrasthira;S. Angsubhakorn
摘要 后睾丸病是泰国东北部的一种地方性寄生虫病。该地区寄生虫和胆管癌的同时发生以及此类肿瘤的发病率高于其他地区。亚硝化剂、可亚硝化物质和亚硝胺化合物通常存在于该地区的几种稳定食品中。为了研究寄生虫和亚硝基化合物之间的相互作用，我们将叙利亚金仓鼠分为四组：第一组，未经治疗；第 2 组，二甲基亚硝胺（DMN，0.0025% 或 25 ppm）处理；第3组，100个囊蚴处理；第 4 组，DMN（0.0025% 或 25 ppm）加上 100 个囊蚴治疗组。同时接受 DMN 和寄生虫的动物（第 4 组）出现胆管癌（100%）和胆管纤维化（100%）。尽管在 DMN 组（第 2 组）的一些动物中发现了胆管纤维化，但在接受 DMN（第 2 组）或单独寄生虫（第 3 组）的组中未观察到肿瘤。据推测，这些动物中胆管癌的发生是因为 DMN 对受寄生虫刺激的胆管上皮细胞的增殖改变产生了致癌作用。这些发现表明，DMN 摄入和肝吸虫感染的结合可能在人类肝内导管肿瘤的致癌过程中发挥重要作用。
Abstract Opisthorchiasis is an endemic parasitic disease in northeastern Thailand. The concomitant occurrence of the parasite and cholangiocarcinoma as well as the incidence of such tumors are higher in that part of the country than in other areas. Nitrosating agents, nitrosatable substances, and nitrosamine compounds are commonly present in several kinds of stable foods in that region. To study the interactions between the parasite and the nitroso compounds, we divided Syrian golden hamsters into four groups: Group 1, untreated; Group 2, dimethylnitrosamine (DMN, 0.0025% or 25 ppm)-treated; Group 3, 100 metacercariae-treated; and Group 4, DMN (0.0025% or 25 ppm) plus 100 metacercariae-treated groups. The animals that received both DMN and parasites (Group 4) developed cholangiocarcinoma (100%) and cholangiofibrosis (100%). The tumor was not observed in the group that received either DMN (Group 2) or parasites alone (Group 3), although cholangiofibrosis was found in some animals in the DMN group (Group 2). It is postulated that cholangiocarcinomas in these animals arose because the DMN exerted a carcinogenic effect on the altered proliferating epithelial cells of bile ducts that had been stimulated by the parasite. These findings suggest that the combination of DMN ingestion and liver fluke infestation may play an important role in the carcinogenesis of the intrahepatic duct neoplasms in human beings.