Programmable assembly of nanoarchitectures using genetically engineered viruses
Programmable assembly of nanoarchitectures using genetically engineered viruses
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DOI：
10.1021/nl050795d
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发表时间：
2005-07-01
期刊：
影响因子：
10.8
通讯作者：
Belcher, AM
中科院分区：
文献类型：
--
作者：
Huang, Y;Chiang, CY;Belcher, AM
生物系统具有固有的分子识别和自组装能力，是构建具有分子精度的复杂材料结构的诱人模板。在这里，我们报告了各种纳米结构的组装，包括纳米颗粒阵列、异纳米颗粒体系结构和纳米线，使用高度工程的M13噬菌体作为模板。M13噬菌体基因组可以被合理地设计成产生具有丝状衣壳和末端表达的不同底物特异性多肽的病毒颗粒，为复杂纳米结构的可编程组装提供通用模板。构建了衣壳上有金结合基序、链霉亲和素一端有链霉亲和素结合基序的噬菌体克隆，并用于将金和镉纳米晶组装成有序的一维阵列和更复杂的几何结构。初步研究表明，这种纳米颗粒阵列还可以作为模板，成核高导电纳米线，这对寻址/互连单个纳米结构非常重要。
Biological systems possess inherent molecular recognition and self-assembly capabilities and are attractive templates for constructing complex material structures with molecular precision. Here we report the assembly of various nanoachitectures including nanoparticle arrays, hetero-nanoparticle architectures, and nanowires utilizing highly engineered M13 bacteriophage as templates. The genome of M13 phage can be rationally engineered to produce viral particles with distinct substrate-specific peptides expressed on the filamentous capsid and the ends, providing a generic template for programmable assembly of complex nanostructures. Phage clones with gold-binding motifs on the capsid and streptavidin-binding motifs at one end are created and used to assemble Au and Cdse nanocrytals into ordered one-dimensional arrays and more complex geometries. Initial studies show such nanoparticle arrays can further function as templates to nucleate highly conductive nanowires that are important for addressing/interconnecting individual nanostructures.