AMEBA-TO-FLAGELLATE TRANSFORMATION IN TETRAMITUS ROSTRATUS .2. MICROTUBULAR MORPHOGENESIS
AMEBA-TO-FLAGELLATE TRANSFORMATION IN TETRAMITUS ROSTRATUS .2. MICROTUBULAR MORPHOGENESIS
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DOI：
10.1083/jcb.35.2.323
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发表时间：
1967-01-01
影响因子：
7.8
通讯作者：
KLUSS, BC
中科院分区：
文献类型：
--
作者：
OUTKA, DE;KLUSS, BC
Tetramitus 表现出独立的变形虫和鞭毛虫阶段，具有显着的形态二分性。阿米巴的转化涉及 4 个动体及其鞭毛的形成。这些 Mnetosomes 和相关的微管螺旋在薄膜下延伸的排列形成了不对称的鞭毛形式。虽然在阿米巴中没有发现可识别的运动体前体，并且没有迹象表明分裂鞭毛虫具有自我复制，但已经确定了运动体的发育阶段。这些偶尔与细胞核或位于原动体近端内部或附近的致密体相关。鞭毛的生长涉及轴丝和膜的形成。从运动体微管的远端长成一个短芽，它很快形成一个膨胀的气球，其中含有细小的珠状丝网。微管的自由端似乎散开；它们首先被视为单个元素，然后被视为双联体，最后被排列成圆柱体。长度的增长伴随着球囊直径的减小。提出了这样的概念：动力装置的形成可能涉及核贡献，随后是微管的自发组装。
Tetramitus exhibits independent ameboid and flagellate stages of remarkable morphological dichotomy. Transformation of the ameba involves the formation of 4 kinetosomes and their flagella. The arrangement of these Mnetosomes and associated whorls of microtubules extending under the pellicle establishes the asymmetric flagellate form. While no recognizable kinetosomal precursors have been seen in amebae, and there is no suggestion of self-replication in dividing flagellates, developmental stages of kinetosomes have been identified. These are occasionally seen in association with the nucleus or with dense bodies which lie either inside of or close to the proximal end of the prokinetosome. Outgrowth of flagella involves formation of an axoneme and a membrane. From the distal tip of the kinetosome microtubules grow into a short bud, which soon forms an expanded balloon containing a reticulum of finely beaded filaments. The free ends of the microtubules appear unraveled; they are seen first as single elements, then as doublets, and finally are arranged into a cylinder. Growth in length is accompanied by a reduction in the diameter of the balloon. The concept that the formation of the kinetic apparatus might involve a nuclear contribution, followed by a spontaneous assembly of microtubules, is suggested.