Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides
Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides
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DOI：
10.1021/jacs.7b08087
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发表时间：
2017-10-11
影响因子：
15
通讯作者：
Ke, Yonggang
中科院分区：
文献类型：
--
作者：
Jiang, Tao;Meyer, Travis A.;Ke, Yonggang
我们描述了两种不同的建筑单位：胶原蛋白模拟肽和DNA折纸的共同组装。设计了两种肽CP++和sCP（++），其序列包含中心嵌段（Pro-Hyp-Gly）和在N-和C-末端的两个带正电荷的结构域（Pro-Arg-Gly）。肽和DNA折纸双层（TL）纳米片的共组装提供了具有类似于10 nm的重复周期的一维纳米线的形成。结构分析表明，一个面对面的堆叠的DNA纳米片与肽对齐垂直的表表面。我们证明了潜在的选择性肽-DNA协会之间的面对面和边到边包装定制的DNA纳米结构的大小。这项研究提出了一种有吸引力的策略，从肽和基于DNA的构建块中创建混合生物分子组装体，该构建块利用各自组分的固有化学和物理性质来编码易于获得的仿生材料中的结构和潜在的功能复杂性。
We describe the co-assembly of two different building units: collagen-mimetic peptides and DNA origami. Two peptides CP++ and sCP(++) are designed with a sequence comprising a central block (Pro-Hyp-Gly) and two positively charged domains (Pro-Arg-Gly) at both N- and C-termini. Co-assembly of peptides and DNA origami two-layer (TL) nanosheets affords the formation of one-dimensional nanowires with repeating periodicity of similar to 10 nm. Structural analyses suggest a face-to-face stacking of DNA nanosheets with peptides aligned perpendicularly to the sheet surfaces. We demonstrate the potential of selective peptide-DNA association between face-to-face and edge-to-edge packing by tailoring the size of DNA nanostructures. This study presents an attractive strategy to create hybrid biomolecular assemblies from peptide and DNA-based building blocks that takes advantage of the intrinsic chemical and physical properties of the respective components to encode structural and, potentially, functional complexity within readily accessible biomimetic materials.