High-Throughput Detection and Sizing of Individual Low-Index Nanoparticles and Viruses for Pathogen Identification
High-Throughput Detection and Sizing of Individual Low-Index Nanoparticles and Viruses for Pathogen Identification
复制标题
DOI：
10.1021/nl103210p
复制
发表时间：
2010-11-01
期刊：
影响因子：
10.8
通讯作者：
Uenlue, M. S.
中科院分区：
文献类型：
--
作者：
Daaboul, G. G.;Yurt, A.;Uenlue, M. S.
对生物制剂进行快速、芯片级且具有成本效益的单颗粒检测对人类健康和国家安全至关重要。我们报道了对单个低折射率聚苯乙烯纳米颗粒以及甲型H1N1流感病毒的实时、高通量检测及尺寸测定。我们的宽场共路干涉仪能在非常大的传感区域检测纳米颗粒和病毒，比竞争技术的传感区域大几个数量级。我们证明了能够检测到直径小至70纳米的纳米颗粒并测定其尺寸。我们可以清晰地区分直径为70、100、150和200纳米的纳米颗粒的尺寸。我们还在一次实验中展示了对数百个单个甲型H1N1流感病毒的检测及尺寸特征分析。
Rapid, chip-scale, and cost-effective single particle detection of biological agents is of great importance to human health and national security. We report real-time, high-throughput detection and sizing of individual, low-index polystyrene nanoparticles and HI NI virus. Our widefield, common path interferometer detects nanoparticles and viruses over a very large sensing area, orders of magnitude larger than competing techniques. We demonstrate nanoparticle detection and sizing down to 70 nm in diameter. We clearly size discriminate nanoparticles with diameters of 70, 100, 150, and 200 nm. We also demonstrate detection and size characterization of hundreds of individual H1N1 viruses in a single experiment.