Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment.
Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment.
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DOI：
10.1099/ijs.0.64500-0
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发表时间：
2007
影响因子：
2.8
通讯作者：
Xiang Xiao;Peng Wang;Xiang Zeng;D. Bartlett;Fengping Wang
中科院分区：
文献类型：
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作者：
Xiang Xiao;Peng Wang;Xiang Zeng;D. Bartlett;Fengping Wang
对从西太平洋深海沉积物中分离到的两株希瓦氏菌（Shewanella-like bacteria）WP 2（T）和WP 3（T）进行了分类学研究。两种细菌的细胞均为兼性厌氧、革兰氏阴性杆菌，并通过单个极鞭毛运动。菌株WP 2（T）是嗜冷的，最佳生长在约10-15 ℃，而菌株WP 3（T）是耐冷的，最佳生长在15-20 ℃。两株菌在0.1 ~ 50 MPa的压力范围内生长，最适生长压力为20 MPa。菌株WP 3（T）能够利用硝酸盐、富马酸盐、N-氧化三甲胺（TMAO）、DMSO和不溶性Fe（III）作为末端电子受体，而菌株WP 2（T）只能利用硝酸盐、TMAO和DMSO作为末端电子受体。菌株WP 2（T）和WP 3（T）的16 S rRNA基因序列的同源性为97%，并且分别与希瓦氏菌KMM 3589和海底希瓦氏菌ATCC 43992（T）的16 S rRNA基因序列的同源性最高（97%）。WP 2（T）和WP 3（T）的gyrB基因序列与紫色希瓦氏菌（Shewanellaviolacea）JCM 10179（T）和赛氏希瓦氏菌（Shewanellassairae）SM 2 -1（T）的相似性最高，分别为90%和87%。与16 SrRNA基因序列分析结果相反，基于gyrB基因序列分析的遗传学分析将菌株WP 2（T）、S. violacea和S. WP 3（T）与S. sports和S. sairae。DNA-DNA杂交结果支持菌株WP 2（T）与S. violacea和S.海底生物。系统发育证据以及DNA-DNA相关性和表型特征表明，这两个新菌株代表了两个新的深海希瓦氏菌物种。拟命名为嗜冷希瓦氏菌（Shewanella psychrophila sp. nov.）（模式菌株WP 2（T）= JCM 13876（T）=CGMCC 1.6159（T））和耐压希瓦氏菌（Shewanella piezotolerans）（模式菌株WP 3（T）=JCM 13877（T）=CGMCC 1.6160（T））。
Two Shewanella-like bacterial strains, WP2(T) and WP3(T), which were isolated from west Pacific deep-sea sediment, were studied to determine their taxonomic position. Cells of the two bacteria were facultatively anaerobic, Gram-negative rods and motile by means of a single polar flagellum. Strain WP2(T) was psychrophilic, growing optimally at about 10-15 degrees C, whereas strain WP3(T) was psychrotolerant, growing optimally at 15-20 degrees C. The two strains grew in the pressure range 0.1-50 MPa, with optimal growth at 20 MPa. Strain WP3(T) was able to use nitrate, fumarate, trimethylamine N-oxide (TMAO), DMSO and insoluble Fe(III) as terminal electron acceptors during anaerobic growth, whereas strain WP2(T) was able to use only nitrate, TMAO and DMSO. The 16S rRNA gene sequences of strains WP2(T) and WP3(T) were 97 % identical, and showed highest similarity (97 %) to those of Shewanella fidelis KMM 3589 and Shewanella benthica ATCC 43992(T), respectively. The gyrB gene sequences of strains WP2(T)and WP3 (T) were also determined, and showed highest similarity to those of Shewanella violacea JCM 10179(T) (90 %) and Shewanella sairae SM2-1(T) (87 %), respectively. Contrary to the 16S rRNA gene sequence results, the phylogeny based on gyrB gene sequence analysis placed strain WP2(T), S. violacea and S. benthica in one group, while strain WP3(T) grouped with S. fidelis and S. sairae. DNA-DNA hybridization experiments supported the placement of strain WP2(T) with S. violacea and S. benthica. Phylogenetic evidence, together with DNA-DNA relatedness and phenotypic characteristics, indicated that the two new strains represented two novel deep-sea Shewanella species. The names Shewanella psychrophila sp. nov. (type strain WP2(T)=JCM 13876(T)=CGMCC 1.6159(T)) and Shewanella piezotolerans (type strain WP3(T)=JCM 13877(T)=CGMCC 1.6160(T)) are proposed.