Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs)
Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs)
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DOI：
10.1021/ma501530c
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发表时间：
2014-09
期刊：
影响因子：
5.5
通讯作者：
A. Poma;Heli A. Brahmbhatt;J. Watts;N. Turner
中科院分区：
文献类型：
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作者：
A. Poma;Heli A. Brahmbhatt;J. Watts;N. Turner
MIP NPs是目前分子识别和诊断应用中最合适的抗体替代品之一。本文首次采用修饰的可聚合的2 ' -脱氧腺苷互补残基制备了2 ' -脱氧腺苷（dA）印迹MIP NPs。我们证明，这种改性单体的引入增加了所生产的MIP NPs的亲和力，而不改变其物理性质，如大小、形状或分散性。印迹的NPs显示出特异性识别dA的能力，而对2 ' -脱氧鸟苷（dG）或脱氧胞苷（dC）没有印迹作用。结果表明，这些单体（及其磷酰胺）可用于合成低聚物或更长的DNA序列，从而有可能生产具有更高识别性能的杂交MIP-DNA材料。
MIP NPs represent one of the current most suitable alternatives to antibodies for molecular recognition and diagnostic applications. Here we present the synthesis of MIP NPs imprinted for 2′-deoxyadenosine (dA) prepared using for the first time a modified polymerizable 2′-deoxyuridine complementary residue. We demonstrate that the introduction of this modified monomer results in an increase of the affinity of the produced MIP NPs, without altering their physical properties such as size, shape, or dispersibility. The imprinted NPs have shown the ability to specifically recognize dA, whereas no imprinting effect was observed for 2′-deoxyguanosine (dG) or deoxycytidine (dC). The results suggest that such monomers (and their phosphoramidites) could be used in the synthesis of oligomer or longer DNA sequences for potentially producing hybrid MIP–DNA materials with improved recognition performance.