Electronic 'off-on' molecular switch for rapid detection of thrombin
Electronic 'off-on' molecular switch for rapid detection of thrombin
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DOI：
10.1002/elan.200603610
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发表时间：
2006-10-01
期刊：
影响因子：
3
通讯作者：
O'Sullivan, Ciara K.
中科院分区：
文献类型：
--
作者：
Lluis Acero Sanchez, Josep;Baldrich, Eva;O'Sullivan, Ciara K.
为特征。将该凝血酶结合适配体的双功能衍生物固定在Au电极表面，该衍生物长15个碱基，具有氧化还原活性的二茂铁片段和在适配体链末端的垂坠的己硫醇连接基团。采用循环伏安法（CV）、计时安培法（CA）、计时电位法（CP）、方波伏安法（SWV）和计时电位溶出分析（PSA）等技术研究了该传感器的电化学行为。综上所述，这些实验支持当凝血酶与固定的适体结合时，系统的开关“打开”机制。在HEPES缓冲液（pH 8.0, 0.01 M）中，采用方波伏安法和时间电位溶出法对靶蛋白凝血酶浓度的响应变化进行了评价，结果表明，该传感器在1.0 ~ 35 nM范围内对凝血酶有特异性响应，检测限为亚纳摩尔水平。对该传感器的反应时间、重现性、特异性和稳定性进行了研究。
characterized. A bifunctional derivative of the thrombin-binding aptamer, 15-base long with a redox-active ferrocene moiety and a pendant hexanethiol linker group at the termini of the aptamer strand was immobilized on Au electrode surface. The electrochemical behavior of the aptasensor was studied using cyclic voltammetry (CV), chronoamperometry (CA), chronopotentiometry (CP), square-wave voltammetry (SWV) and chronopotentiometric stripping analysis (PSA) techniques. Taken together, these experiments support the switch "on" mechanism of the system when the thrombin binds to the immobilized aptamer. The variation of response to the concentration of thrombin, the target protein, was evaluated by square-wave voltammetry and chronopotentiometric stripping analysis in HEPES buffer solution (pH 8.0, 0.01 M). ne aptasensor showed a specific response to thrombin in the range 1.0 to 35 nM with a detection limit of subnanomolar level concentrations. The reaction time, reproducibility, specificity and stability of the aptasensor were also studied.