PURIFICATION OF CELL-WALL PEPTIDOGLYCAN OF DIMORPHIC BACTERIUM CAULOBACTER-CRESCENTUS
PURIFICATION OF CELL-WALL PEPTIDOGLYCAN OF DIMORPHIC BACTERIUM CAULOBACTER-CRESCENTUS
复制标题
DOI：
10.1016/0003-9861(75)90094-6
复制
发表时间：
1975-01-01
影响因子：
3.9
通讯作者：
SHEDLARSKI, JG
中科院分区：
文献类型：
--
作者：
GOODWIN, SD;SHEDLARSKI, JG
已制备了二型细菌新月柄杆菌CB 2的细胞血影。游动细胞和柄状细胞的幽灵保留了营养细胞的个体形态。血影中唯一存在的糖是肽聚糖的特征性糖，即，N-乙酰胞壁酸和N-乙酰葡糖胺。对血影中存在的主要氨基酸的摩尔比的定量表明，尽管有柄细胞以类似于其他革兰氏阴性细菌的肽聚糖组成的比例（1：1.7：0.94）含有谷氨酸、丙氨酸和二氨基庚二酸，但群集细胞肽聚糖缺乏二氨基庚二酸，并且与有柄细胞肽聚糖相比仅含有一半量的丙氨酸。这些相同的氨基酸在分裂前细胞的摩尔比是中间的那些发现在蜂群和柄细胞。
Cell ghosts of the dimorphic bacterium,Caulobacter crescentusCB2, have been prepared. Ghosts of the swarmer and stalked cells retain the individual morphology of the vegetative cells. The only sugars present in the ghosts are those characteristic of peptidoglycan, i.e.,N-acetylmuramic acid andN-acetylglucosamine. Quantitation of the molar ratios of the principle amino acids present in the ghosts demonstrates that whereas the stalked cell contains glutamic acid, alanine, and diaminopimelic acid in a ratio (1:1.7:0.94) similar to the peptidoglycan composition of other Gram-negative bacteria, the swarmer cell peptidoglycan lacks diaminopimelic acid and contains only one-half the amount of alanine compared to stalked cell peptidoglycan. Molar ratios of these same amino acids in the predivisional cell are intermediate to those found in the swarmer and stalked cells.