CA-125 IN NORMAL-TISSUES AND CARCINOMAS OF THE UTERINE CERVIX, ENDOMETRIUM AND FALLOPIAN-TUBE .1. IMMUNOHISTOCHEMICAL DETECTION
CA-125 IN NORMAL-TISSUES AND CARCINOMAS OF THE UTERINE CERVIX, ENDOMETRIUM AND FALLOPIAN-TUBE .1. IMMUNOHISTOCHEMICAL DETECTION
复制标题
DOI：
10.1007/bf00931381
复制
发表时间：
1989-01-01
影响因子：
2.6
通讯作者：
BOLTE, A
中科院分区：
文献类型：
--
作者：
SCHARL, A;CROMBACH, G;BOLTE, A
癌抗原125（CA 125）的分布已被调查的正常组织和Muellerian管癌的免疫组化方法使用单克隆抗体OC 125。CA 125的检测在低温切片中最强烈，在福尔马林固定和石蜡包埋的组织中根据固定时间而降低。用神经氨酸酶或碱水解酶消化消除了特异性染色，表明抗原是通过碱不稳定键与蛋白质结合的唾液酸糖。免疫组化结果显示CA 125在正常子宫颈内膜、子宫内膜和输卵管腺上皮中均有不同程度的表达。正常子宫内膜细胞分布与月经周期有关。在子宫颈、子宫内膜和输卵管腺癌中，73%的病例发现CA 125。在腺体结构中，抗原集中在肿瘤细胞的腔表面，在实体瘤区域中，抗原分布在整个细胞质中或集中在大的细胞质空泡中。CA 125在实体瘤区域的表达显著降低。这些数据表明CA 125不是真正的“肿瘤标志物”，而是女性生殖器粘膜及其癌性衍生物的产物，只要它们的合成能力在病理分化过程中没有丧失。
The distribution of cancer antigen 125 (CA 125) has been investigated in normal tissues and carcinomas of the Muellerian duct by immunohistochemical methods using the monoclonal antibody OC 125. Detection of CA 125 was most intense in cryostat sections and decreased in formalin fixed and paraffin embedded tissues according to the duration of fixation. Enzymatic digestion with neuraminidase or alkaline hydrolysis abolished specific staining suggesting the antigen is a sialylsaccharide bound to protein by alkali-labile linkage. Immunohistochemical staining demonstrated the presence of CA 125 in all normal glandular epithelial of the endocervix, endometrium and fallopian tube in different distribution patterns. In normal endometrium the cellular distribution pattern was related to the menstrual cycle. In endocervical, endometrial and tubal adenocarcinomas CA 125 was found in 73% of cases. In glandular structures the antigen was concentrated at the luminal surface of the tumour cells, in solid tumour areas it was spread throughout the cytoplasm or concentrated in large cytoplasmic vacuoles. The expression of CA 125 was considerably lower in solid tumour areas. These data show that CA 125 is not a true "tumour marker", but a product of female genital mucosae and of their cancerous derivates provided their synthesizing ability is not lost in the course of pathologic differentiation.