Effect of peroxides on [3H]D-aspartate release from bovine isolated retinae.
Effect of peroxides on [3H]D-aspartate release from bovine isolated retinae.
复制标题
过氧化物对牛离体视网膜释放[3H]D-天冬氨酸的影响。
DOI：
10.1023/b:nere.0000018855.77589.5d
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发表时间：
2004
影响因子：
4.4
通讯作者：
Ohia,SunnyE
中科院分区：
文献类型：
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作者：
LeDay,AngelaM;Awe,SundayO;Kulkarni,Kaustubh;Harris,LydiaC;Opere,Catherine;Dash,Alekha;Ohia,SunnyE
在本研究中，我们研究了天然存在的和合成的过氧化物对K+-去极化诱发的牛离体视网膜释放[3 H] d-天冬氨酸的影响。此外，过氧化物对内源性谷氨酸浓度的影响，测定了在牛神经视网膜和玻璃体液的眼睛与过氧化氢（H2 O2）离体。天然存在的H2 O2（1-100 μM）和合成（氢过氧化枯烯，cuOOH; 1-100 μM）过氧化物均引起K+诱发的[3 H] d-天冬氨酸释放的浓度依赖性抑制，而不影响基础氚外排。抗氧化剂trolox（2 mM）可阻止H2 O2（30 μM）和CuOOH（10 μM）引起的[3 H] d-天冬氨酸溢出抑制。3-氨基-三唑（3- AT 100 mM）抑制过氧化氢酶可增强低浓度H2 O2（1 μM）的抑制作用，但可拮抗H2 O2（30 μM）对K+诱导的[3 H] d-天冬氨酸释放的影响。在Inex体内实验中，外源性应用H2 O2（1-100 μM）也导致牛视网膜中谷氨酸水平的浓度相关性降低。我们的结论是，过氧化物可以抑制K+-诱发释放的[3 H] d-天冬氨酸，也降低内源性谷氨酸浓度在牛视网膜。
In the present study, we investigated the effect of naturally occurring and synthetic peroxides on K+-depolarization-evoked release of [3H]d-aspartate from bovine isolated retinae. Furthermore, effect of peroxides on endogenous glutamate concentrations were measured by HPLC in bovine neural retinae and vitreous humor of eyes treated with hydrogen peroxide (H2O2)ex vivo. Both naturally occurring H2O2(1–100 μM) and synthetic (cumene hydroperoxide, cuOOH; 1–100 μM) peroxides caused a concentration-dependent inhibition of K+-evoked [3H]d-aspartate release without affecting basal tritium efflux. The antioxidant, trolox (2 mM) prevented the inhibition of evoked [3H]d-aspartate overflow elicited by both H2O2(30 μM) and cuOOH (10 μM). Inhibition of catalase by 3-amino-triazole (3- AT 100 mM) enhanced an inhibitory effect of a low concentration of H2O2(1 μM) but antagonized the effect of H2O2(30 μM) on K+-induced [3H]d-aspartate release. Inex vivoexperiments, exogenously applied H2O2(1–100 μM) also caused a concentration-related decrease in glutamate levels in the bovine retina. We conclude that peroxides can inhibit K+-evoked release of [3H]d-aspartate and also decrease endogenous glutamate concentrations in the bovine retina.