Immunohistochemical demonstration of tubulin associated with microtubules and synaptic junctions in mammalian brain
Immunohistochemical demonstration of tubulin associated with microtubules and synaptic junctions in mammalian brain
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哺乳动物大脑中与微管和突触连接相关的微管蛋白的免疫组织化学演示
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
Sanjida Mughal
中科院分区：
文献类型：
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作者：
A. Matus;B. B. Walters;Sanjida Mughal
微管蛋白在脑组织中的分布已通过光镜和电镜免疫组织化学研究，使用抗血清提出了对神经学纯化的微管衍生的微管蛋白。当用非免疫血清代替抗微管蛋白血清时，染色的缺乏和用抗微管蛋白以外的抗原的抗血清获得的完全不同的染色表明了染色的特异性。抗微管蛋白免疫组化显示微管蛋白抗原存在于微管和大多数突触的突触后连接晶格中。在突触间隙或轴突末端均未检测到抗原。这种分布证实了以前的生化鉴定微管蛋白作为一个主要的突触后连接的组成部分，但表明它是不常见的所有突触连接。
SummaryThe distribution of tubulin in brain tissue has been investigated by light and electron microscopic immunohistochemistry using an antiserum raised against electrophoretically purified microtubule-derived tubulin. The specificity of the staining is indicated by the lack of staining when non-immune serum is substituted for anti-tubulin serum and the quite different staining obtained with antisera against antigens other than tubulin. The anti-tubulin immunohistochemistry reveals tubulin antigen in microtubules and in the postsynaptic junctional lattice of most synapses. No antigen has been detected in either the synaptic cleft or in the axon terminal. This distribution confirms previous biochemical identification of tubulin as a major postsynaptic junctional component, but suggests that it is not common to all synaptic junctions.