Morphological characterization of Etv2 vascular explants using fractal analysis and atomic force microscopy.
Morphological characterization of Etv2 vascular explants using fractal analysis and atomic force microscopy.
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使用分形分析和原子力显微镜对 Etv2 血管外植体进行形态学表征。
DOI：
10.1016/j.mvr.2021.104205
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发表时间：
2021-11
影响因子：
3.1
通讯作者：
Rabbany SY
中科院分区：
文献类型：
--
作者：
Adelson RP;Palikuqi B;Weiss Z;Checco A;Schreiner R;Rafii S;Rabbany SY
血管网络的快速植入是组织外植体功能整合的关键。然而，现有的诱导血管生成方法使用的方法产生的血管时间稳定性差或机械完整性不足，从而降低了它们在体内的稳健性。转录因子Ets变体2 （Etv2）指定胚胎造血和血管内皮细胞（EC）的发育，并在出生后血管再生和肿瘤血管生成过程中短暂地重新激活。本研究探讨了Etv2上调在体外和体内形成稳定血管床中的作用。对照、Etv2+胎源性人脐静脉内皮细胞（HUVECs）和成人脐静脉内皮细胞在血管床上血管新生生长。利用分形维数和空隙度对这些血管床进行了表征，分别量化了它们的分支复杂性和空间填充均匀性。原子力显微镜（AFM）用于探索是否更大的复杂性和均匀性导致更机械稳定的血管。此外，使用EC完整性标记来探索机制线索。Etv2+ HUVECs表现出更大的分支，血管密度和结构均匀性，体外和体内刚度降低，表明更倾向于稳定的血管形成。与结肠肿瘤类器官组织共培养时，与单独培养的Etv2+ HUVECs相比，Etv2+ HUVECs的分形维数和腔隙度降低，表明由于Etv2的存在，血管密度和血管间距的均匀性增加。本研究提出了一个新的概念，即分形维数、空隙度和AFM与传统的血管生成测量（包括血管分支和密度）一样，可以用来评估血管灌注和稳定性。
The rapid engraftment of vascular networks is critical for functional incorporation of tissue explants. However, existing methods for inducing angiogenesis utilize approaches that yield vasculature with poor temporal stability or inadequate mechanical integrity, which reduce their robustness in vivo. The transcription factor Ets variant 2 (Etv2) specifies embryonic hematopoietic and vascular endothelial cell (EC) development, and is transiently reactivated during postnatal vascular regeneration and tumor angiogenesis. This study investigates the role for Etv2 upregulation in forming stable vascular beds both in vitro and in vivo. Control and Etv2+ prototypical fetal-derived human umbilical vein ECs (HUVECs) and adult ECs were angiogenically grown into vascular beds. These vessel beds were characterized using fractal dimension and lacunarity, to quantify their branching complexity and space-filling homogeneity, respectively. Atomic force microscopy (AFM) was used to explore whether greater complexity and homogeneity lead to more mechanically stable vessels. Additionally, markers of EC integrity were used to probe for mechanistic clues. Etv2+ HUVECs exhibit greater branching, vessel density, and structural homogeneity, and decreased stiffness in vitro and in vivo, indicating a greater propensity for stable vessel formation. When co-cultured with colon tumor organoid tissue, Etv2+ HUVECs have decreased fractal dimension and lacunarity compared to Etv2+ HUVECs cultured alone, indicating that vessel density and homogeneity of vessel spacing increased due to the presence of Etv2. This study sets forth the novel concept that fractal dimension, lacunarity, and AFM are as informative as conventional angiogenic measurements, including vessel branching and density, to assess vascular perfusion and stability.
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DOI：
10.1111/j.1549-8719.2010.00075.x
发表时间：
2011-02
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Gould DJ;Vadakkan TJ;Poché RA;Dickinson ME
通讯作者：
Dickinson ME
间隙连接介导的内皮细胞刚度调节。
影响因子：
4.6
作者：
Okamoto T;Kawamoto E;Takagi Y;Akita N;Hayashi T;Park EJ;Suzuki K;Shimaoka M
通讯作者：
Shimaoka M
影响因子：
16.6
作者：
Frye M;Taddei A;Dierkes C;Martinez-Corral I;Fielden M;Ortsäter H;Kazenwadel J;Calado DP;Ostergaard P;Salminen M;He L;Harvey NL;Kiefer F;Mäkinen T
通讯作者：
Mäkinen T
影响因子：
20.3
作者：
Carmona, Guillaume;Goettig, Stephan;Chavakis, Emmanouil
通讯作者：
Chavakis, Emmanouil
影响因子：
4
作者：
Oberleithner, Hans;Riethmueller, Christoph;Schillers, Hermann
通讯作者：
Schillers, Hermann