Circulating MicroRNAs as Novel Biomarkers of Stenosis Progression in Asymptomatic Carotid Stenosis
Circulating MicroRNAs as Novel Biomarkers of Stenosis Progression in Asymptomatic Carotid Stenosis
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DOI：
10.1161/strokeaha.116.013650
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发表时间：
2017-01-01
期刊：
影响因子：
8.3
通讯作者：
Lago, Aida
中科院分区：
文献类型：
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作者：
Dolz, Sandra;Gorriz, David;Lago, Aida
背景和目的：管腔狭窄> 50%的无症状颈动脉狭窄（ACAS）的进展被认为是缺血性卒中的潜在危险因素;然而，缺乏ACAS进展的亚临床分子生物标志物。最近的研究表明，一些microRNA（miRNAs）的调节功能的颈动脉斑块的演变，但其在ACAS进展的作用大多是未知的。我们研究的目的是调查ACAS患者外周血外泌体中的一个广泛的miRNA组，以将循环miRNA表达谱与狭窄进展相关联。首先，通过来自该队列的16名患者的血浆样品的Affyellow微阵列确定循环外泌体的miRNA表达谱。第二，ACAS进展患者中最差异表达的miRNAs通过实时聚合酶链反应在一个单独的复制队列中39例患者sample.Results-Our结果显示，ACAS进展与卒中的发展相关。miR-199 b-3 p、miR-27 b3 p、miR-130 a-3 p、miR-221- 3 p和miR-24- 3 p在ACAS进展患者中的表达显著升高。结论：总之，我们的研究支持特定循环miRNA表达谱可以提供一种新的工具，补充ACAS进展的监测，改善预防缺血性卒中的治疗方法。
Background and Purpose-Progression of asymptomatic carotid artery stenosis (ACAS) in patients with > 50% luminal narrowing is considered a potential risk factor for ischemic stroke; however, subclinical molecular biomarkers of ACAS progression are lacking. Recent studies suggest a regulatory function for several microRNAs (miRNAs) on the evolution of carotid plaque, but its role in ACAS progression is mostly unknown. The aim of our study was to investigate a wide miRNA panel in peripheral blood exosomes from patients with ACAS to associate circulating miRNA expression profiles with stenosis progression.Methods-The study included 60 patients with ACAS carrying > 50% luminal narrowing. First, miRNA expression profiles of circulating exosomes were determined by Affymetrix microarrays from plasma samples of 16 patients from the cohort. Second, those miRNAs among the most differentially expressed in patients with ACAS progression were quantified by real-time polymerase chain reaction in a separate replication cohort of 39 subjects within the patient sample.Results-Our results showed that ACAS progression was associated with development of stroke. MiR-199b-3p, miR-27b3p, miR-130a-3p, miR-221-3p, and miR-24-3p presented significant higher expression in those patients with ACAS progression.Conclusions-In conclusion, our study supports that specific circulating miRNA expression profiles could provide a new tool that complements the monitoring of ACAS progression, improving therapeutic approaches to prevent ischemic stroke.