Role of contraction in the structure and growth of neonatal rat cardiocytes.
Role of contraction in the structure and growth of neonatal rat cardiocytes.
复制标题
收缩在新生大鼠心肌细胞结构和生长中的作用。
DOI：
10.1152/ajpheart.1987.253.6.h1391
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Lauva,IK
中科院分区：
文献类型：
--
作者：
Marino,TA;Kuseryk,L;Lauva,IK
本研究的目的是确定收缩在调节新生大鼠心肌细胞生长大小中的作用。为了达到这个目的，实验在四组心肌细胞上进行：1)附着在底物上的静止心肌细胞，2)附着在底物上的收缩心肌细胞，3)不附着在底物上的静止心肌细胞，以及4)不附着在底物上的收缩心肌细胞。心肌细胞在无血清和添加血清的培养基中生长1周，并评估心肌细胞的表面积、体积、数量和精细结构。这项研究最重要的结果是，附着在基质上并受刺激收缩的心肌细胞体积增大。然而，在确定的无血清培养基中，单独收缩或附着于底物本身都不能导致新生儿心肌细胞的大小增长。另一个重要的发现是，其他非特异性生长促进剂，如血清中发现的那些，在附着于底物的收缩心肌细胞中，刺激心肌细胞大小更大幅度的增长。
The aim of this study was to determine the role of contraction in the regulation of neonatal rat cardiocyte growth in size. To accomplish this objective, experiments were done on four groups of cardiocytes: 1) quiescent cardiocytes attached to a substrate, 2) contracting cardiocytes attached to a substrate, 3) quiescent cardiocytes not attached to a substrate, adn 4) contracting cardiocytes not attached to a substrate. The cardiocytes were grown in both serum-free and serum-supplemented media for up to 1 wk, and cardiocyte surface area, volume, number, and fine structure were evaluated. The most important result of this study was that cardiocytes, which are attached to a substrate and stimulated to contract, grow in size. However, neither contraction alone nor attachment to a substrate by itself resulted in neonatal cardiocyte growth in size in defined serum-free medium. Another important finding was that other nonspecific growth promoters, such as those found in serum, stimulated more substantial growth in cardiocyte size in contracting cardiocytes that were attached to a substrate.