Light field microscopy
Light field microscopy
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DOI：
10.1145/1141911.1141976
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发表时间：
2006-07-01
影响因子：
6.2
通讯作者：
Horowitz, Mark
中科院分区：
文献类型：
--
作者：
Levoy, Marc;Ng, Ren;Horowitz, Mark
通过在传统显微镜的光学序列中插入微透镜阵列，人们可以在一张照片中捕捉生物标本的光场。虽然衍射对这些光场的空间和角度分辨率的乘积有限制，但我们仍然可以从中产生有用的透视视图和焦点堆栈。由于显微镜是固有的正射影设备，透视视图代表了一种观察显微标本的新方法。从一张照片中创建焦点堆栈的能力允许记录移动或光敏标本。对这些焦点堆栈应用3D反卷积，我们可以生成一组横截面，可以使用体绘制将其可视化。在本文中，我们展示了一种原型光场显微镜（LFM），分析了其光学性能，并展示了各种生物标本的透视图，焦点堆栈和重建体积。我们还表明，合成聚焦后进行三维反褶积相当于直接对四维光场进行有限角层析成像。
By inserting a microlens array into the optical train of a conventional microscope, one can capture light fields of biological specimens in a single photograph. Although diffraction places a limit on the product of spatial and angular resolution in these light fields, we can nevertheless produce useful perspective views and focal stacks from them. Since microscopes are inherently orthographic devices, perspective views represent a new way to look at microscopic specimens. The ability to create focal stacks from a single photograph allows moving or light-sensitive specimens to be recorded. Applying 3D deconvolution to these focal stacks, we can produce a set of cross sections, which can be visualized using volume rendering. In this paper, we demonstrate a prototype light field microscope (LFM), analyze its optical performance, and show perspective views, focal stacks, and reconstructed volumes for a variety of biological specimens. We also show that synthetic focusing followed by 3D deconvolution is equivalent to applying limited-angle tomography directly to the 4D light field.