Extreme-value analysis of intracellular cargo transport by motor proteins
Extreme-value analysis of intracellular cargo transport by motor proteins
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DOI：
10.1038/s42005-024-01538-4
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发表时间：
2024-02-13
影响因子：
5.5
通讯作者：
Hayashi，Kumiko
中科院分区：
文献类型：
--
作者：
Naoi，Takuma;Kagawa，Yuki;Hayashi，Kumiko
极值分析（伊娃）处理数据与概率分布中值的偏差。伊娃服务于各种目的，如预测灾害和分析体育记录。在此，我们扩展了伊娃的使用，以研究纳米运动蛋白在活蠕虫秀丽隐杆线虫神经元中的运动功能。马达蛋白，如驱动蛋白和动力蛋白，分别沿着微管沿着顺行和逆行运动，以运送细胞所需的含货物的物质。虽然从平均速度值无法推断出两种电机之间的本质差异，但从速度数据获得的返回水平伊娃图显示了差异。伊娃顺行和逆行传输的广义极值分布的形状参数。我们的研究结果扩展了伊娃用于分析体内纳米级蛋白质运动数据的可能性和适用性。这一结果已作为预印本出版。
Extreme-value analysis (EVA) deals with deviations in the data from the median of the probability distributions. EVA serves various purposes such as predicting disasters and analyzing sports records. Herein, we extended the use of EVA to investigate the motility functions of nanoscale motor proteins in neurons of the living worm Caenorhabditis elegans. Motor proteins, such as kinesin and dynein, move along microtubules anterogradely and retrogradely, respectively, to deliver the cargo-containing materials needed for the cells. Although the essential difference between the two motors could not be inferred from the mean velocity values, the return-level EVA plots obtained from the velocity data revealed a difference. Shape parameters of the generalized extreme value distribution of EVA for anterograde transport and for retrograde transport. Our findings extend the possibility and applicability of EVA for analyzing motility data of nanoscale proteins in vivo. This result has been published as a preprint.