Fluorescence Detection of Single Guest Molecules in Ultrasmall Droplets of Non polar Solvent
Fluorescence Detection of Single Guest Molecules in Ultrasmall Droplets of Non polar Solvent
复制标题
非极性溶剂超小液滴中单个客体分子的荧光检测
DOI：
10.1039/c1cp22207d
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
H.Miyasaka
中科院分区：
文献类型：
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作者：
M.Yasuda,A.Iida;S.Ito;H.Miyasaka
利用共聚焦和宽场显微镜技术研究了N，N′-二丙基-1，6，7，12-四（4-叔丁基苯氧基）-3，4，9，10-二萘嵌苯四羧酸二酰亚胺（BP-PDI）在正辛烷超小液滴中的发光行为。小液滴中的单个BP-PDI在荧光强度的时间过程中没有显示出可区分的闪烁。这归因于导致荧光关闭状态的长寿命电离状态的形成的小概率。荧光偏振度和宽场荧光图像的时间变化表明，在正辛烷和含水环境之间的界面处的荧光分子的短时间吸附。荧光相关光谱分析表明，吸附/脱附过程至少发生在两个不同的时间尺度上，这可能是由于染料与界面之间的吸附几何构型和/或相互作用（如货车范德华力和氢键）的不同所致.
We have investigated emissive behaviours of individual perylenebisimide derivatives, N,N′-dipropyl-1,6,7,12-tetrakis(4-tert-butylphenoxy)-3,4,9,10-perylenetetra-carboxydiimide (BP-PDI), in single ultrasmall droplets of n-octane at room temperature by using confocal and wide-field microscopic techniques. Single BP-PDIs in the small droplets show no distinguishable blinking in the time courses of fluorescence intensity. This is attributed to small probabilities of the formation of the long-lived ionized state leading to the off-state of the fluorescence. Temporal change in the degree of polarization of fluorescence and wide-field fluorescence images indicated short-time adsorption of the fluorescent molecules at the interfaces between n-octane and watery environments. Fluorescence correlation spectroscopy revealed that the adsorption/desorption processes took place at least in two different time scales, probably due to the difference in the adsorption geometry and/or in the interaction, such as van der Waals interaction and hydrogen bonding, between the dye and the interface.