Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid
Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid
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一小部分人卵泡液诱导人精子顶体反应
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
S. Meizel
中科院分区：
文献类型：
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作者：
S. Suarez;D. Wolf;S. Meizel
将人类射出的精子通过Percoll梯度洗涤，在含有血清白蛋白的限定培养基中预孵育10小时，然后通过加入人类卵泡液或Sephadex G-75柱部分的流体诱导经历快速顶体反应。诱导卵泡液没有发生时，精子预孵育仅为0或5小时。使用针对人精子顶体区域的单克隆抗体的间接免疫荧光检测的反应。透射电镜计数的顶体丢失百分率与免疫荧光计数的顶体丢失百分率一致。含有顶体反应诱导活性峰的Sephadex G-75组分的表观分子量为45，000 ± 4，200（SD）。生理性顶体反应的发生得到以下支持：评估运动性（当精子与牛血清白蛋白预孵育时，在处理期间未发生显著的活力损失），透射电子显微镜（符合顶体反应的超微结构标准），和去透明带仓鼠卵母细胞结合和穿透（用卵泡液的活性部分预处理，然后洗涤并与卵母细胞一起孵育的精子显示出显著更大的与卵母细胞的结合和穿透）。卵泡液刺激顶体反应显然不是由于血清污染;用来自卵泡液供体的血清处理预孵育的精子对精子没有影响。目前正在研究该馏分中活性组分的性质。
Human ejaculated spermatozoa were washed through a Percoll gradient, preincubated for 10 hr in a defined medium containing serum albumin, and then induced to undergo rapid acrosome reactions by addition of human follicular fluid or a Sephadex G-75 column fraction of the fluid. Induction by follicular fluid did not occur when the spermatozoa were preincubated for only 0 or 5 hr. The reactions were detected by indirect immunofluorescence using a monoclonal antibody directed against the human sperm acrosomal region. The percentage of acrosomal loss counted by transmission electron microscopy agreed with that counted by immunofluorescence. The apparent molecular weight of the Sephadex G-75 fraction containing the peak of acrosome reaction-inducing activity was 45,000 ± 4,200 (SD). The occurrence of physiological acrosome reactions was supported by: assessing motility (no significant loss of motility occurred during the treatment period when sperm were preincubated with bovine serum albumin), transmission electron microscopy (the ultrastructural criteria for the acrosome reaction were met), and zona-free hamster oocyte binding and penetration (spermatozoa pretreated with the active fraction of follicular fluid, then washed and incubated with oocytes, showed significantly greater binding to and penetration of oocytes). The stimulation of the acrosome reaction by follicular fluid is apparently not due to blood serum contamination; treatment of preincubated spermatozoa with sera from the follicular fluid donors had no effect on the spermatozoa. The nature of the active component(s) in that fraction is currently being investigated.