Growth Factors from Tumor Microenvironment Possibly Promote the Proliferation of Glioblastoma-Derived Stem-like Cells in Vitro
Growth Factors from Tumor Microenvironment Possibly Promote the Proliferation of Glioblastoma-Derived Stem-like Cells in Vitro
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DOI：
10.1007/s12253-012-9543-7
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发表时间：
2012-10-01
影响因子：
2.8
通讯作者：
Liao, Hong
中科院分区：
文献类型：
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作者：
Guo, JingJing;Niu, Rui;Liao, Hong
多形性胶质母细胞瘤是一种恶性胶质瘤，具有生存率低、复发率高的特点，近年来的研究表明，胶质母细胞瘤干细胞是其发病的重要原因。肿瘤组织中的微环境或小生境被认为是肿瘤干细胞异常生长的重要支持。为探讨肿瘤微环境中生长因子对胶质母细胞瘤干细胞行为的影响，用抗表面标志物CD 133抗体从成人胶质母细胞瘤标本中分离胶质母细胞瘤源性干细胞样细胞（GDSCs），并与U87 MG细胞、未分选胶质母细胞瘤细胞、CD 133（-）细胞和正常大鼠原代星形胶质细胞共培养。结果表明，肿瘤细胞可以促进GDSC增殖，而非肿瘤细胞则不能，并且在与肿瘤细胞的共培养系统中仅检测到多种生长因子。结论：来源于肿瘤微环境的生长因子可能导致GDSCs的不受控制的增殖。
Glioblastoma multiform is a lethal brain glial tumor characterized by low survival and high recurrence, partially attributed to the glioblastoma stem cells according to recent researches. Microenvironment or niche in tumor tissue is believed to provide essential support for the aberrant growth of tumor stem cells. In order to explore the effect of growth factors in tumor microenvironment on glioblastoma stem cells behavior, glioblastoma-derived stem-like cells (GDSCs) were isolated from adult human glioblastoma specimen with antibody against surface marker CD133 and were co-cultured with various tumor cells including U87MG cells, unsorted glioblastoma tumor cells, CD133(-) cells and normal rat primary astrocytes. Results suggested that tumor cells could promote GDSCs proliferation while non-tumor cells could not, and several growth factors were exclusively detected in the co-culture system with tumor cells. It was concluded that growth factors derived from tumor microenvironment possibly contributed to the uncontrolled proliferation of GDSCs.