Greater numbers of human spermatozoa associate with endosalpingeal cells derived from the isthmus compared with those from the ampulla
Greater numbers of human spermatozoa associate with endosalpingeal cells derived from the isthmus compared with those from the ampulla
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DOI：
10.1093/humrep/12.9.1985
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发表时间：
1997-09-01
影响因子：
6.1
通讯作者：
Barratt, CLR
中科院分区：
文献类型：
--
作者：
Baillie, HS;Pacey, AA;Barratt, CLR
一个简单的共培养生物测定系统被用来调查是否或不解剖来源影响上皮细胞的能力，从人类子宫本研究还通过比较不同的组织培养模型和评估雌二醇浓度的影响，将从人输卵管不同区域收获的上皮外植体与已知浓度的活动供体精子共孵育。所有结果均经过调整以反映标准精子浓度5x 10（6）/ml，与壶腹上皮相比，峡部每个视野结合的精子更多[峡部9.5 +/- 0.9，壶腹7.1 +/- 0.7（平均值+/- SEM）; n = 36，P < 0.05，ANOVA]，绝经后患者的细胞结合精子的能力明显降低[峡部5.5 ± 2.0，壶腹4.3 +/- 1.4（平均值+/- SEM）; n = 4]，月经周期阶段和添加周期中期浓度的17 β-雌二醇（750 pmol/l）均不影响结合到输卵管区域上皮的精子数量。此外，每个视野结合极化外植体的精子数高于结合分离的原代和传代上皮细胞单层的精子数（P < 0.05）。这份报告是第一个提供的证据表明，精子上皮细胞结合的作用，在形成一个峡精子水库在人类输卵管，结果还表明，雌激素不参与这些相互作用的调节，细胞极性是一个重要因素，这种协会在体外。
A simple co-culture bioassay system was used to investigate whether or not the anatomical origin affected the ability of epithelial cells from the human uterine (Fallopian) tube to 'bind' spermatozoa, This study was also used to identify some of the factors which may be involved in the regulation of sperm-epithelial interactions in vitro by comparing different tissue culture models and assessing the effect of oestradiol concentration, Epithelial explants harvested from different regions of human uterine tubes were coincubated with a known concentration of motile donor spermatozoa. All results were adjusted to reflect a standard sperm concentration of 5x10(6)/ml, More spermatozoa associated per field of isthmic compared to ampullary epithelium [isthmus 9.5 +/- 0.9, ampulla 7.1 +/- 0.7 (mean +/- SEM); n = 36, P < 0.05, ANOVA] and cells from postmenopausal patients had an apparently reduced ability to bind spermatozoa [isthmus 5.5 +/- 2.0, ampulla 4.3 +/- 1.4 (mean +/- SEM); n = 4], Neither menstrual cycle stage nor addition of mid-cycle concentrations of 17 beta-oestradiol (750 pmol/l) affected the number of spermatozoa which bound to epithelium from either tubal region. In addition, the number of spermatozoa which bound per field of polarized explants was greater (P < 0.05) than that bound to dissociated primary and passaged epithelial cell monolayers. This report is the first to provide evidence suggestive of a role for sperm-epithelial binding in the formation of an isthmic sperm reservoir in the human uterine tube, Results also indicate that oestrogen is not involved in the regulation of these interactions, and that cell polarity is an important factor for such associations in vitro.