T-CELL REPERTOIRES IN HEALTHY AND DISEASED HUMAN TISSUES ANALYZED BY T-CELL RECEPTOR BETA-CHAIN CDR3 SIZE DETERMINATION - EVIDENCE FOR OLIGOCLONAL EXPANSIONS IN TUMORS AND INFLAMMATORY DISEASES
T-CELL REPERTOIRES IN HEALTHY AND DISEASED HUMAN TISSUES ANALYZED BY T-CELL RECEPTOR BETA-CHAIN CDR3 SIZE DETERMINATION - EVIDENCE FOR OLIGOCLONAL EXPANSIONS IN TUMORS AND INFLAMMATORY DISEASES
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DOI：
10.1016/0923-2494(96)80240-9
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发表时间：
1995-02-01
期刊：
影响因子：
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通讯作者：
KOURILSKY, P
中科院分区：
文献类型：
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作者：
EVEN, J;LIM, A;KOURILSKY, P
已经报道了在浸润的病变组织中寡克隆T细胞扩增的许多实例。然而，它仍然有待建立这样的观察是否可以推广，并在何种程度上寡克隆模式后，在体外培养的T细胞浸润反映在体内的情况。使用新的高分辨率分析，不需要在体外细胞扩增，我们检测到这样的寡克隆T细胞扩增7/7黑色素瘤肿瘤活检，3/3活检的炎症皮肤在急性移植物抗宿主病（aGVHD）后异基因骨髓移植（alloBMT）和7/7类风湿性关节炎患者的滑膜。因此，当使用足够灵敏的检测方法时，很容易观察到寡克隆T细胞扩增，这表明在不同疾病的T细胞浸润中，相似的扩增是规律。这种观察和监测在疾病过程中和体外培养过程中这种扩增的体内演变应具有重要的临床意义。
Many examples of oligoclonal T-cell expansion in infiltrated diseased tissues have been reported. However, it remains to be established whether such observations can be generalized and to what extent oligoclonal patterns obtained after in vitro culture of T-cell infiltrates reflect in vivo situations. Using new high resolution analysis which requires no in vitro cellular expansion, we detected such oligoclonal T-cell expansions in 7/7 melanoma tumour biopsies, 3/3 biopsies of inflammatory skin during acute graft versus host disease (aGVHD) after allogeneic bone marrow transplantation (alloBMT) and 7/7 synovial membranes from patients with rheumatoid arthritis. Thus, oligoclonal T-cel expansions are readily observed when a sufficiently sensitive detection method is used, suggesting that similar expansions are the rule among T-cell infiltrates in different diseases. This observation and the monitoring of the in vivo evolution of such expansion during the course of the disease and during in vitro culture should have important clinical implications.